{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T08:52:59Z","timestamp":1785747179124,"version":"3.56.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019180","name":"HORIZON EUROPE European Research Council","doi-asserted-by":"crossref","award":["852600"],"award-info":[{"award-number":["852600"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FWO","award":["1SD0622N"],"award-info":[{"award-number":["1SD0622N"]}]},{"DOI":"10.13039\/501100007660","name":"UAntwerp","doi-asserted-by":"crossref","award":["FFI220247"],"award-info":[{"award-number":["FFI220247"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007660","name":"UAntwerp","doi-asserted-by":"crossref","award":["FFB220205"],"award-info":[{"award-number":["FFB220205"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007660","name":"UAntwerp","doi-asserted-by":"crossref","award":["FFI220319"],"award-info":[{"award-number":["FFI220319"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,12]]},"abstract":"<jats:p>A novel strain of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5320\" xlink:type=\"simple\">Lactobacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, named AMBV1719<jats:sup>T<\/jats:sup>, was isolated from the vagina of a healthy participant in our large-scale citizen science project on the female microbiome, named Isala. Phylogenetic analysis showed that the 16S rRNA gene of AMBV1719<jats:sup>T<\/jats:sup> is most similar to that of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.14549\" xlink:type=\"simple\">Lactobacillus taiwanensis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> with a sequence similarity of 99.873\u200a%. However, a genome-wide comparison using average nucleotide identity (ANI) revealed that isolate AMBV1719<jats:sup>T<\/jats:sup> showed the highest ANI with <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.33067\" xlink:type=\"simple\">Lactobacillus paragasseri<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> JCM 5343<jats:sup>T<\/jats:sup>, with a value of only 88.17\u200a%. This low ANI value with the most closely related strains known to date indicated that AMBV1719<jats:sup>T<\/jats:sup> represents a distinct species. This strain has a limited ability to degrade carbon sources compared to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5372\" xlink:type=\"simple\">Lactobacillus gasseri<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, indicating its adaptation to the host. Its genome has a length of 2.12\u2009Mb with a G+C content of 34.8\u2009mol%. We thus propose the name <jats:italic>Lactobacillus isalae<\/jats:italic> sp. nov. for this novel species, with AMBV1719<jats:sup>T<\/jats:sup> (=LMG 32886<jats:sup>T<\/jats:sup>=CECT 30756<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006038","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T13:17:42Z","timestamp":1697116662000},"source":"Crossref","is-referenced-by-count":10,"title":["Lactobacillus isalae sp. nov., isolated from the female reproductive tract"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7509-2902","authenticated-orcid":false,"given":"Tom","family":"Eilers","sequence":"first","affiliation":[{"name":"Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, 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