{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:45:39Z","timestamp":1789429539318,"version":"build-2803163510"},"reference-count":64,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"NRF KOREA","award":["2021R1A2C3004015"],"award-info":[{"award-number":["2021R1A2C3004015"]}]},{"name":"NRF KOREA","award":["PJ01700703"],"award-info":[{"award-number":["PJ01700703"]}]},{"name":"NRF KOREA","award":["NIBR202325101"],"award-info":[{"award-number":["NIBR202325101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,12]]},"abstract":"Strain 16-5T<\/jats:sup>, a mesophilic methanotroph of the genus \n \n Methylococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, was isolated from rice field soil sampled in Chungcheong Province, Republic of Korea. Strain 16-5T<\/jats:sup> had both particulate and soluble methane monooxygenases and could only grow on methane and methanol as electron donors. Strain 16-5\u2009T<\/jats:sup> cells are Gram-negative, white to light tan in color, non-motile, non-flagellated, diplococcoid to cocci, and have the typical type I intracytoplasmic membrane system. Strain 16-5T<\/jats:sup> grew at 18\u201338\u200a\u00b0C (optimum, 27\u200a\u00b0C) and at pH 5.0\u20138.0 (optimum, pH 6.5\u20137.0). C16\u200a:\u200a1<\/jats:sub>\u2009\u03c97c<\/jats:italic> (38.8%), C16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>5c<\/jats:italic> (18.8%), C16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>6c<\/jats:italic> (16.8%) and C16\u200a:\u200a0<\/jats:sub> (16.9%) were the major fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid were the major polar lipids. The main respiratory quinone was methylene-ubiquinone-8. Strain 16-5T<\/jats:sup> displayed the highest 16S rRNA gene sequence similarities to other taxonomically recognized members of the genus \n \n Methylococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, i.e. \n \n Methylococcus capsulatus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> TexasT<\/jats:sup> (98.62%) and \n \n Methylococcus geothermalis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> IM1T<\/jats:sup> (98.49\u200a%), which were its closest relatives. It did, however, differ from all other taxonomically described \n \n Methylococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species due to some phenotypic differences, most notably its inability to grow at temperatures above 38\u200a\u00b0C, where other \n \n Methylococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species thrive. Its 4.34 Mbp-sized genome has a DNA G+C content of 62.47\u2009mol%, and multiple genome-based properties such as average nucleotide identity and digital DNA-DNA hybridization value distanced it from its closest relatives. Based on the data presented above, this strain represents the first non-thermotolerant species of the genus \n \n Methylococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The name Methylococcus mesophilus<\/jats:italic> sp. nov. is proposed, and 16-5T<\/jats:sup> (=JCM 35359T<\/jats:sup>=KCTC 82050T<\/jats:sup>) is the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006077","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T16:17:56Z","timestamp":1697127476000},"source":"Crossref","is-referenced-by-count":7,"title":["Methylococcus mesophilus sp. nov., the first non-thermotolerant methanotroph of the genus Methylococcus, from a rice field"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2553-1604","authenticated-orcid":false,"given":"Samuel Imisi","family":"Awala","sequence":"first","affiliation":[{"name":"Department of Microbiology, Chungbuk National University, Cheongju 28644, Republic of 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