{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:07:06Z","timestamp":1785514026496,"version":"3.56.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"10","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["91851203"],"award-info":[{"award-number":["91851203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science and Technology Fundamental Resources Investigation Program of China","award":["2021FY100900"],"award-info":[{"award-number":["2021FY100900"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,13]]},"abstract":"<jats:p>Two Gram-stain-positive, facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated CLL-3-40<jats:sup>T<\/jats:sup> and CLL-7-23, were isolated from coastal sediment sampled in Changyi, Shandong Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains were related to the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4857\" xlink:type=\"simple\">Bacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and close to six type strains of species within the <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4935\" xlink:type=\"simple\">Bacillus licheniformis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> group. In phenotypic characterization tests, strain CLL-3-40<jats:sup>T<\/jats:sup> could grow at 15\u201350\u200a\u00b0C (optimum, 37\u200a\u00b0C) and in media with pH 5\u20139 (optimum pH 7.0), and tolerate up to 12\u200a% (w\/v) NaCl. The fermentation broth supernatant extracted by ethyl acetate of strain CLL-3-40<jats:sup>T<\/jats:sup> could inhibit aquaculture pathogenic vibrios. The predominant cellular fatty acids of strain CLL-3-40<jats:sup>T<\/jats:sup> were anteiso-C<jats:sub>15\u200a:\u200a0<\/jats:sub> (30.7\u200a%) and iso-C<jats:sub>15\u200a:\u200a0<\/jats:sub> (31.5\u200a%); the peptidoglycan from cell-wall contained <jats:italic>meso<\/jats:italic>-diaminopimelic acid; the predominant quinone was menaquinone 7; and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and two unidentified phospholipids. The digital DNA\u2013DNA hybridization values and average nucleotide identities among strains CLL-3-40<jats:sup>T<\/jats:sup> and CLL-7-23 and their close type strains were less than 21.9 and 48.4\u200a%, respectively, thereby indicating that strain CLL-3-40<jats:sup>T<\/jats:sup> should represent a novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4857\" xlink:type=\"simple\">Bacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The genomic DNA G+C contents were 38.4\u200amol% in strain CLL-3-40<jats:sup>T<\/jats:sup> and 38.3\u200amol% in strain CLL-7-23. The 12 biosynthetic gene clusters of strain CLL-3-40<jats:sup>T<\/jats:sup> were predicted based on results from the online server antiSMASH. Based upon the consensus of phenotypic and genotypic results, strain CLL-3-40<jats:sup>T<\/jats:sup> should be classified as representing a novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4857\" xlink:type=\"simple\">Bacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, for which the name <jats:italic>Bacillus changyiensis<\/jats:italic> sp. nov. is proposed. The type strain is CLL-3-40<jats:sup>T<\/jats:sup> (= MCCC 1A14857<jats:sup>T<\/jats:sup>=JCM 35755<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006089","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T13:16:02Z","timestamp":1697202962000},"source":"Crossref","is-referenced-by-count":3,"title":["Bacillus changyiensis sp. nov., isolated from coastal sediment"],"prefix":"10.1099","volume":"73","author":[{"given":"Guiqian","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Biology, College of Science, Shantou University, Shantou, Guangdong 515063, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiliang","family":"Lai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyong","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6066-7013","authenticated-orcid":false,"given":"Zongze","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Shantou University, Shantou, Guangdong 515063, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.018879-0"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-3-1305"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-2-256"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.050682-0"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13690"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/462645"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-11-9"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-9000-0"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001755"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1007\/BF01734359"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00161174"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-018-8014-6"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab335"},{"key":"R20","volume-title":"Determinative Manual for Routine Bacteriology","author":"Dong","year":"2001"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-100-2-221"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80158-X"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/am.28.2.226-231.1974"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-4-463"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1021\/np500424y"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.595991"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2017.12.018"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002007"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000441"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000462"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-5-1671"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64029-0"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.006089?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T13:16:07Z","timestamp":1697202967000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.006089"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,13]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10,13]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.006089","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,13]]}}}