{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:34:19Z","timestamp":1787942059551,"version":"build-2784847793"},"reference-count":20,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"crossref","award":["P4-0092"],"award-info":[{"award-number":["P4-0092"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,25]]},"abstract":"During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species in Slovenia, five \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-like strains were cultured from caeca of a total of 104 domestic pigs that could not be identified using the standard-prescribed biochemical tests or MALDI-TOF MS. The isolates were obtained using the standard ISO 10272 procedure for the isolation of thermotolerant \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with prolonged cultivation time. Small \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-like colonies were observed on mCCDA and CASA agar plates after 2\u20134\u2009days of incubation; dark-field microscopy revealed relatively big spirilli-shaped bacteria exhibiting characteristic \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-like motility. The cells were 1.5\u20133\u2009\u00b5m long and 0.5\u20130.7\u2009\u00b5m wide, Gram-negative, oxidase-positive and catalase-positive. MALDI-TOF mass spectra were distinctive and consistent, but with low MALDI-TOF MS log scores and the closest matches being those of \n \n Campylobacter hyointestinalis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Campylobacter fetus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. All five strains underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed that the isolates were most similar (98.3\u201398.4\u200a% identity) to \n \n Campylobacter lanienae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Pairwise average nucleotide identity (ANI) values revealed that the five studied strains shared pairwise ANI of 96.2\u201396.5\u200a% but were clearly distinct from the previously described \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species (ANI \u226472.8\u200a%). The core genome-based phylogeny confirmed that the new strains form a distinct and well-supported clade within the genus \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The conducted polyphasic taxonomic analysis confirmed that the five strains represent a novel \n \n Campylobacter<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species for which the name Campylobacter magnus<\/jats:italic> sp. nov. is suggested, with strain 46386T<\/jats:sup> (=DSM 115534T<\/jats:sup>=CCUG 76865T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006108","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T14:16:59Z","timestamp":1698243419000},"source":"Crossref","is-referenced-by-count":8,"title":["Campylobacter magnus sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4862-2090","authenticated-orcid":false,"given":"Igor","family":"Gruntar","sequence":"first","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rok","family":"Kostanj\u0161ek","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Biotechnical Faculty, Department of Biology, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Pir\u0161","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bojan","family":"Papi\u0107","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002255"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004332"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-1605(02)00317-3"},{"key":"R4","article-title":"OIE Terrestrial Manual 2021. 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