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Cells of strains FTZ2<jats:sup>T<\/jats:sup> and FTZ6<jats:sup>T<\/jats:sup> were cocci, with diameters of 0.6\u20131.0\u2009\u00b5m and 0.6\u20130.9\u2009\u00b5m, respectively. Both strains grew on methanol, methylamine, dimethylamine and trimethylamine, but not on acetate, formate, H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, choline, betaine or dimethyl sulphide. Strain FTZ2<jats:sup>T<\/jats:sup> grew at 10\u201337\u200a\u00b0C (optimally at 33\u200a\u00b0C), pH 5.5\u20138.0 (optimally at pH 7.0) and 0\u20131.03 M NaCl (optimally at 0.17 M NaCl). In contrast, strain FTZ6<jats:sup>T<\/jats:sup> grew at 15\u201342\u200a\u00b0C (optimally at 37\u200a\u00b0C), pH 5.0\u20137.5 (optimally pH 6.5) and 0\u20131.03 M NaCl (optimally at 0.17 M NaCl). Both strains required magnesium for growth and were susceptible to sodium dodecyl sulphate. Biotin was required for the growth of strain FTZ2<jats:sup>T<\/jats:sup> but not of strain FTZ6<jats:sup>T<\/jats:sup>. The genomic G+C contents of strains FTZ2<jats:sup>T<\/jats:sup> and FTZ6<jats:sup>T<\/jats:sup> were 41.6 and 40.9\u200amol%, respectively. Phylogenetic analyses revealed that strain FTZ2<jats:sup>T<\/jats:sup> was mostly related to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31598\" xlink:type=\"simple\">Methanolobus psychrotolerans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> YSF-03<jats:sup>T<\/jats:sup>, with 16S rRNA gene similarity of 98.6\u200a%, an average nucleotide identity (ANI) of 82.5\u200a%, and a digital DNA\u2013DNA hybridization (dDDH) of 24.6\u200a%. While strain FTZ6<jats:sup>T<\/jats:sup> was mostly related to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.257\" xlink:type=\"simple\">Methanolobus vulcani<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> PL-12\/M<jats:sup>T<\/jats:sup>, with 16S rRNA gene similarity of 99.4\u200a%, an ANI of 88.6% and a dDDH of 34.6\u200a%. Based on phenotypic, phylogenetic and genotypic evidence, two novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.251\" xlink:type=\"simple\">Methanolobus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, <jats:italic>Methanolobus mangrovi<\/jats:italic> sp. nov. and <jats:italic>Methanolobus sediminis<\/jats:italic> sp. nov., are proposed. The type strain of <jats:italic>M. mangrovi<\/jats:italic> sp. nov. is FTZ2<jats:sup>T<\/jats:sup> (=CCAM 1276<jats:sup>T<\/jats:sup>=JCM 39396<jats:sup>T<\/jats:sup>) and the type strain of <jats:italic>M. sediminis<\/jats:italic> sp. nov. is FTZ6<jats:sup>T<\/jats:sup> (=CCAM 1277<jats:sup>T<\/jats:sup>=JCM 39397<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006169","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T10:16:35Z","timestamp":1700475395000},"source":"Crossref","is-referenced-by-count":6,"title":["Methanolobus mangrovi sp. nov. and Methanolobus sediminis sp. nov, two novel methylotrophic methanogens isolated from mangrove sediments in China"],"prefix":"10.1099","volume":"73","author":[{"given":"Jinjie","family":"Zhou","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong, PR China"},{"name":"Archaeal 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