{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:40:23Z","timestamp":1775176823609,"version":"3.50.1"},"reference-count":27,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2020R1F1A1066895"],"award-info":[{"award-number":["NRF-2020R1F1A1066895"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,24]]},"abstract":"<jats:p>An obligate anaerobic, Gram-negative, rod-shaped and non-spore-forming bacterium, designated as strain GYB001<jats:sup>T<\/jats:sup>, was isolated from the blood of a patient with a sigmoid colon perforation. Taxonomic characterization of the novel isolate was performed using a polyphasic approach. A phylogenetic analysis based on 16S rRNA gene and whole genome sequences revealed that GYB001<jats:sup>T<\/jats:sup> represented a member of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10183\" xlink:type=\"simple\">Parabacteroides<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, in the family <jats:italic>\n                     <jats:named-content content-type=\"family\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.36475\" xlink:type=\"simple\">Tannerellaceae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The closest species, based on 16S rRNA sequence, was <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.14864\" xlink:type=\"simple\">Parabacteroides gordonii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 23371<jats:sup>T<\/jats:sup> with 97.4\u200a% similarity. Average nucleotide identity and digital DNA\u2013DNA hybridization values between strain GYB001<jats:sup>T<\/jats:sup> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.14864\" xlink:type=\"simple\">P. gordonii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 23371<jats:sup>T<\/jats:sup> were 86.7 and 28.7% and between GYB001<jats:sup>T<\/jats:sup> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.26616\" xlink:type=\"simple\">Parabacteroides faecis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> JCM 18682<jats:sup>T<\/jats:sup> were 86.6 and 27.7\u200a%, respectively. The genome was 6.57 Mbp long with 43.3\u2009mol% G+C content. Colonies on Brucella blood agar (BBA) were circular, convex, smooth, grey and small in size. Growth was observed on trypticase soy agar (TSA), TSA +5\u200a% sheep blood and <jats:italic>Euglena gracilis<\/jats:italic> agar. Growth occurred at 18\u201342\u200a\u00b0C on BBA in the presence of 0\u20133\u200a%\u2009NaCl (w\/v) and at pH 6.0\u20138.5. The major polar lipids were phosphatidylethanolamine and phospholipids. The major fatty acids in strain GYB001<jats:sup>T<\/jats:sup> were anteiso-C<jats:sub>15\u200a:\u200a0<\/jats:sub> and iso-C<jats:sub>17\u200a:\u200a0<\/jats:sub> 3-OH, and the predominant respiratory quinones were menaquinone-10 (MK-10) and MK-9. The cell wall contained <jats:italic>meso<\/jats:italic>-diaminopimelic acid. Considering these phenotypic features and comparative genome analyses, we propose strain GYB001<jats:sup>T<\/jats:sup> as the type strain of <jats:italic>Parabacteroides leei<\/jats:italic> sp. nov. (=KCTC 25738<jats:sup>T<\/jats:sup>=KBN12P06525<jats:sup>T<\/jats:sup>=LMG 32797<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006187","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T17:17:36Z","timestamp":1700846256000},"source":"Crossref","is-referenced-by-count":4,"title":["Parabacteroides leei sp. nov., isolated from human blood"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5909-5807","authenticated-orcid":false,"given":"Jung-Hyun","family":"Byun","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Gyeongsang National University College of Medicine, Gyeongsang National University Hospital, Jinju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3017-5026","authenticated-orcid":false,"given":"Hae Weon","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Myongji Hospital, Goyang, Republic of Korea"},{"name":"Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8523-4126","authenticated-orcid":false,"given":"Hyukmin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3913-2265","authenticated-orcid":false,"given":"Woo-Kon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gyeongsang National University College of Medicine, Jinju, Republic of 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