{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:30:56Z","timestamp":1773786656023,"version":"3.50.1"},"reference-count":45,"publisher":"Microbiology Society","issue":"1","funder":[{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"crossref","award":["FK 134439"],"award-info":[{"award-number":["FK 134439"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry for Innovation and Technology of Hungary","award":["\u00daNKP-22-3-II New National Excellence Programme"],"award-info":[{"award-number":["\u00daNKP-22-3-II New National Excellence Programme"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,5]]},"abstract":"<jats:p>A Gram-stain-negative strain, designated as D2M1<jats:sup>T<\/jats:sup> was isolated from xylene-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain D2M1<jats:sup>T<\/jats:sup> belongs to the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1783\" xlink:type=\"simple\">Acidovorax<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with the highest 16S rRNA gene similarity to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1790\" xlink:type=\"simple\">Acidovorax delafieldii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 64<jats:sup>T<\/jats:sup> (99.93\u200a%), followed by <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.22659\" xlink:type=\"simple\">Acidovorax radicis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 23535<jats:sup>T<\/jats:sup> (98.77\u200a%) and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31705\" xlink:type=\"simple\">Acidovorax kalamii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> MTCC 12652<jats:sup>T<\/jats:sup> (98.76\u200a%). The draft genome sequence of strain D2M1<jats:sup>T<\/jats:sup> is 5.49\u2009Mb long, and the G+C content of the genome is 64.2\u200amol%. Orthologous average nucleotide identity and digital DNA\u2013DNA hybridization relatedness values between strain D2M1<jats:sup>T<\/jats:sup> and its closest relatives were below the threshold values for species demarcation confirming that strain D2M1<jats:sup>T<\/jats:sup> is distinctly separated from its closest relatives. The whole genome analysis of the strain revealed a phenol degradation gene cluster, encoding a multicomponent phenol hydroxylase (mPH) together with a complete <jats:italic>meta<\/jats:italic>-cleavage pathway including an I.2.C-type catechol 2,3-dioxygenase (C23O) gene. The strain was able to degrade benzene and ethylbenzene as sole sources of carbon and energy under aerobic and microaerobic conditions. Cells were facultatively aerobic rods and motile with a single polar flagellum. The predominant fatty acids (&gt;10\u200a% of the total) of strain D2M1<jats:sup>T<\/jats:sup> were summed feature 3 (C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>\/C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>6<jats:italic>c<\/jats:italic>), C<jats:sub>16\u200a:\u200a0<\/jats:sub> and summed feature 8 (C<jats:sub>18\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>\/C<jats:sub>18\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>6<jats:italic>c<\/jats:italic>). The major ubiquinone of strain D2M1<jats:sup>T<\/jats:sup> was Q8, while the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on polyphasic data, it is concluded that strain D2M1<jats:sup>T<\/jats:sup> represents a novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1783\" xlink:type=\"simple\">Acidovorax<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, for which the name of <jats:italic>Acidovorax benzenivorans<\/jats:italic> sp. nov. is proposed. The type strain of the species is strain D2M1<jats:sup>T<\/jats:sup> (=DSM 115238<jats:sup>T<\/jats:sup>=NCAIM B.02679<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006219","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T15:17:19Z","timestamp":1704467839000},"source":"Crossref","is-referenced-by-count":8,"title":["Acidovorax benzenivorans sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from a xylene-degrading enrichment culture"],"prefix":"10.1099","volume":"74","author":[{"given":"Anna","family":"Bedics","sequence":"first","affiliation":[{"name":"Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, G\u00f6d\u00f6ll\u0151, 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