{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T04:59:16Z","timestamp":1768885156705,"version":"3.49.0"},"reference-count":40,"publisher":"Microbiology Society","issue":"1","funder":[{"name":"National Science Foundation","award":["1736255"],"award-info":[{"award-number":["1736255"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,23]]},"abstract":"<jats:p>A bacterial strain, designated NLS-7<jats:sup>T<\/jats:sup>, was isolated through enrichment of landfill cover soil in methane-oxidizing conditions. Strain NLS-7<jats:sup>T<\/jats:sup> is a Gram-stain negative, non-motile rod, approximately 0.8 \u00b5m wide by 1.3 \u00b5m long. Phylogenetic analysis based on 16S rRNA gene sequencing places it within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1429\" xlink:type=\"simple\">Methylocystis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with its closest relatives being <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.11323\" xlink:type=\"simple\">M. hirsuta<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.42066\" xlink:type=\"simple\">M. silviterrae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.9937\" xlink:type=\"simple\">M. rosea<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with 99.9, 99.7 and 99.6\u200a% sequence similarity respectively. However, average nucleotide identity and average amino acid identity values below the 95\u200a% threshold compared to all the close relatives and digital DNA\u2013DNA hybridization values between 20.9 and 54.1\u200a% demonstrate that strain NLS-7<jats:sup>T<\/jats:sup> represents a novel species. Genome sequencing generated 4.31 million reads and genome assembly resulted in the generation of 244 contigs with a total assembly length of 3\u200a820\u200a957 bp (N50, 37\u200a735 bp; L50, 34). Genome completeness is 99.5\u200a% with 3.98\u200a% contamination. It is capable of growth on methane and methanol. It grows optimally at 30\u200a\u00b0C between pH 6.5 and 7.0. Strain NLS-7<jats:sup>T<\/jats:sup> is capable of atmospheric dinitrogen fixation and can use ammonium (as NH<jats:sub>4<\/jats:sub>Cl), <jats:sc>l<\/jats:sc>-aspartate, <jats:sc>l<\/jats:sc>-arginine, yeast extract, nitrate, <jats:sc>l<\/jats:sc>-leucine, <jats:sc>l<\/jats:sc>-proline, <jats:sc>l<\/jats:sc>-methionine, <jats:sc>l<\/jats:sc>-lysine and <jats:sc>l<\/jats:sc>-alanine as nitrogen sources. The major fatty acids are C<jats:sub>18:1<\/jats:sub> \u03c98<jats:italic>c<\/jats:italic> and C<jats:sub>18:1<\/jats:sub> \u03c97<jats:italic>c<\/jats:italic>. Based upon this polyphasic taxonomic study, strain NLS-7<jats:sup>T<\/jats:sup> represents a novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1429\" xlink:type=\"simple\">Methylocystis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, for which the name <jats:italic>Methylocystis suflitae<\/jats:italic> sp. nov. is proposed. The type strain is NLS-7<jats:sup>T<\/jats:sup> (=ATCC TSD-256<jats:sup>T<\/jats:sup>=DSM 112294<jats:sup>T<\/jats:sup>). The 16S rRNA gene and genome sequences of strain NLS-7<jats:sup>T<\/jats:sup> have been deposited in GenBank under accession numbers ON715489 and GCA_024448135.1, respectively.<\/jats:p>","DOI":"10.1099\/ijsem.0.006239","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T10:54:32Z","timestamp":1706007272000},"source":"Crossref","is-referenced-by-count":5,"title":["Methylocystis suflitae sp. nov., a novel type II methanotrophic bacterium isolated from landfill cover soil"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7871-5581","authenticated-orcid":false,"given":"Christopher T.","family":"Garner","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Oklahoma, 770 Van Vleet Oval, Norman, OK, 73019, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4767-311X","authenticated-orcid":false,"given":"Krithivasan","family":"Sankaranarayanan","sequence":"additional","affiliation":[{"name":"Laboratories of Molecular Anthropology and Microbiome Research, Stephenson Research and Technology Center, University of Oklahoma, 101 David L. 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