{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T11:34:38Z","timestamp":1769859278903,"version":"3.49.0"},"reference-count":60,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["16K07498"],"award-info":[{"award-number":["16K07498"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,13]]},"abstract":"<jats:p>A novel aerobic methanotrophic bacterium, designated as strain IN45<jats:sup>T<\/jats:sup>, was isolated from <jats:italic>in situ<\/jats:italic> colonisation systems deployed at the Iheya North deep-sea hydrothermal field in the mid-Okinawa Trough. IN45<jats:sup>T<\/jats:sup> was a moderately thermophilic obligate methanotroph that grew only on methane or methanol at temperatures between 25 and 56\u200a\u00b0C (optimum 45\u201350\u200a\u00b0C). It was an oval-shaped, Gram-reaction-negative, motile bacterium with a single polar flagellum and an intracytoplasmic membrane system. It required 1.5\u20134.0\u200a% (w\/v) NaCl (optimum 2\u20133\u200a%) for growth. The major phospholipid fatty acids were C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>, C<jats:sub>16\u200a:\u200a0<\/jats:sub> and C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>. The major isoprenoid quinone was Q-8. The 16S rRNA gene sequence comparison revealed 99.1\u200a% sequence identity with <jats:italic>Methylomarinovum caldicuralii<\/jats:italic> IT-9<jats:sup>T<\/jats:sup>, the only species of the genus <jats:italic>Methylomarinovum<\/jats:italic> with a validly published name within the family <jats:italic>Methylothermaceae<\/jats:italic>. The complete genome sequence of IN45<jats:sup>T<\/jats:sup> consisted of a 2.42-Mbp chromosome (DNA G+C content, 64.1\u2009mol%) and a 20.5-kbp plasmid. The genome encodes genes for particulate methane monooxygenase and two types of methanol dehydrogenase (<jats:italic>mxaFI<\/jats:italic> and <jats:italic>xoxF<\/jats:italic>). Genes involved in the ribulose monophosphate pathway for carbon assimilation are encoded, but the transaldolase gene was not found. The genome indicated that IN45<jats:sup>T<\/jats:sup> performs partial denitrification of nitrate to N<jats:sub>2<\/jats:sub>O, and its occurrence was indirectly confirmed by N<jats:sub>2<\/jats:sub>O production in cultures grown with nitrate. Genomic relatedness indices between the complete genome sequences of IN45<jats:sup>T<\/jats:sup> and <jats:italic>M. caldicuralii<\/jats:italic> IT-9<jats:sup>T<\/jats:sup>, such as digital DNA\u2013DNA hybridisation (51.2\u200a%), average nucleotide identity (92.94\u200a%) and average amino acid identity (93.21\u200a%), indicated that these two methanotrophs should be separated at the species level. On the basis of these results, strain IN45<jats:sup>T<\/jats:sup> represents a novel species, for which we propose the name <jats:italic>Methylomarinovum tepidoasis<\/jats:italic> sp. nov. with IN45<jats:sup>T<\/jats:sup> (=JCM 35101<jats:sup>T<\/jats:sup> =DSM 113422<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006288","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T17:17:07Z","timestamp":1710350227000},"source":"Crossref","is-referenced-by-count":2,"title":["Methylomarinovum tepidoasis sp. nov., a moderately thermophilic methanotroph of the family Methylothermaceae isolated from a deep-sea hydrothermal field"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6765-4565","authenticated-orcid":false,"given":"Hisako","family":"Hirayama","sequence":"first","affiliation":[{"name":"Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for 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