{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:25:50Z","timestamp":1784291150514,"version":"3.55.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["3207011"],"award-info":[{"award-number":["3207011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,11]]},"abstract":"<jats:p>With the widespread use of antibiotics, the incidence of antibiotic resistance in microorganisms has increased. <jats:italic>Monochamus alternatus<\/jats:italic> is a trunk borer of pine trees. This study aimed to investigate the <jats:italic>in vitro<\/jats:italic> antimicrobial and biological characteristics of <jats:italic>Enterococcus casseliflavus<\/jats:italic> TN-47 (PP411196), isolated from the gastrointestinal tract of <jats:italic>M. alternatus<\/jats:italic> in Jilin Province, PR China. Among 13 isolates obtained from the insects, five were preliminarily screened for antimicrobial activity. <jats:italic>E. casseliflavus<\/jats:italic> TN-47, which exhibited the strongest antimicrobial activity, was identified. <jats:italic>E. casseliflavus<\/jats:italic> TN-47 possessed antimicrobial activity against <jats:italic>Staphylococcus aureus<\/jats:italic> USA300 and <jats:italic>Salmonella enterica<\/jats:italic> serovar Pullorum ATCC 19945. Furthermore, <jats:italic>E. casseliflavus<\/jats:italic> TN-47 was sensitive to tetracyclines, penicillins (ampicillin, carbenicillin, and piperacillin), quinolones and nitrofuran antibiotics, and resistant to certain beta-lactam antibiotics (oxacillin, cefradine and cephalexin), macrolide antibiotics, sulfonamides and aminoglycosides. <jats:italic>E. casseliflavus<\/jats:italic> TN-47 could tolerate low pH and pepsin-rich conditions in the stomach and grow in the presence of bile acids. <jats:italic>E. casseliflavus<\/jats:italic> TN-47 retained its strong auto-aggregating ability and hydrophobicity. This strain did not exhibit any haemolytic activity. These results indicate that <jats:italic>E. casseliflavus<\/jats:italic> TN-47 has potential as a probiotic. This study provides a theoretical foundation for the future applications of <jats:italic>E. casseliflavus<\/jats:italic> TN-47 and its secondary metabolites in animal nutrition and feed.<\/jats:p>","DOI":"10.1099\/ijsem.0.006305","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T13:18:18Z","timestamp":1712841498000},"source":"Crossref","is-referenced-by-count":2,"title":["Identification and biological characteristics of Enterococcus casseliflavus TN-47 isolated from Monochamus alternatus"],"prefix":"10.1099","volume":"74","author":[{"given":"Zi-Ran","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, 130000, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin 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