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Cells of OY6<jats:sup>T<\/jats:sup> were Gram-stain-negative, pink-pigmented, motile rods and contained an intracytoplasmic membrane structure typical of type I methanotrophs. OY6<jats:sup>T<\/jats:sup> could grow at a pH range of 4.5\u20137.5 (optimum pH 6.5) and at temperatures ranging from 20\u200a\u00b0C to 37\u200a\u00b0C (optimum 30\u200a\u00b0C). The major cellular fatty acids were C<jats:sub>14\u200a:\u200a0<\/jats:sub>, C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>\/C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c96<jats:italic>c<\/jats:italic> and C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c95<jats:italic>c<\/jats:italic>; the predominant respiratory quinone was MQ-8. The genome size was 5.41 Mbp with a DNA G+C content of 51.7\u2009mol%. OY6<jats:sup>T<\/jats:sup> represents a member of the family <jats:italic>Methylococcaceae<\/jats:italic> of the class <jats:italic>Gammaproteobacteria<\/jats:italic> and displayed 95.74\u201399.64\u200a% 16S rRNA gene sequence similarity to the type strains of species of the genus <jats:italic>Methylomonas<\/jats:italic>. Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA\u2013DNA hybridisation (dDDH) confirmed that OY6<jats:sup>T<\/jats:sup> should be classified as representing a novel species. The most closely related type strain was <jats:italic>Methylomonas fluvii<\/jats:italic> EbB<jats:sup>T<\/jats:sup>, with 16S rRNA gene sequence similarity, ANI by <jats:sc>blast<\/jats:sc> (ANIb), ANI by MUMmer (ANIm) and dDDH values of 99.64, 90.46, 91.92 and 44.5\u200a%, respectively. OY6<jats:sup>T<\/jats:sup> possessed genes encoding both the particulate methane monooxygenase enzyme and the soluble methane monooxygenase enzyme. It grew only on methane or methanol as carbon sources. On the basis of phenotypic, genetic and phylogenetic data, strain OY6<jats:sup>T<\/jats:sup> represents a novel species within the genus <jats:italic>Methylomonas<\/jats:italic> for which the name <jats:italic>Methylomonas defluvii<\/jats:italic> sp. nov. is proposed, with strain OY6<jats:sup>T<\/jats:sup> (=GDMCC 1.4114<jats:sup>T<\/jats:sup>=KCTC 8159<jats:sup>T<\/jats:sup>=LMG 33371<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006321","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T14:17:13Z","timestamp":1712931433000},"source":"Crossref","is-referenced-by-count":6,"title":["Methylomonas defluvii sp. nov., a type I methane-oxidizing bacterium from a secondary sedimentation tank of a wastewater treatment 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