{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:22:19Z","timestamp":1781814139728,"version":"3.54.5"},"reference-count":61,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/501100002821","name":"Central Geological Survey, Ministry of Economic Affairs","doi-asserted-by":"crossref","award":["99-5226904000-04-03"],"award-info":[{"award-number":["99-5226904000-04-03"]}],"id":[{"id":"10.13039\/501100002821","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST103\/104\/105\/106\/107-3113-M-005-001"],"award-info":[{"award-number":["MOST103\/104\/105\/106\/107-3113-M-005-001"]}]},{"name":"Sanming University, Sanming, Fujian","award":["20YG09"],"award-info":[{"award-number":["20YG09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,18]]},"abstract":"<jats:p>A novel mesophilic, hydrogenotrophic methanogen, strain CWC-04<jats:sup>T<\/jats:sup>, was obtained from a sediment sample extracted from a gravity core retrieved at station 22 within the KP-9 area off the southwestern coast of Taiwan during the ORIII-1368 cruise in 2009. Cells of strain CWC-04<jats:sup>T<\/jats:sup> were rod-shaped, 1.4\u20132.9\u2009\u00b5m long by 0.5\u20130.6\u2009\u00b5m wide, and occurred singly. Strain CWC-04<jats:sup>T<\/jats:sup>utilized formate, H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, 2-propanol\/CO<jats:sub>2<\/jats:sub> or 2-butanol\/CO<jats:sub>2<\/jats:sub> as catabolic substrates. The optimal growth conditions were 42\u200a\u00b0C, 0.17 M NaCl and pH 5.35. The genomic DNA G+C content calculated from the genome sequence of strain CWC-04<jats:sup>T<\/jats:sup> was 46.19\u2009mol%. Phylogenetic analysis of 16S rRNA gene revealed that strain CWC-04<jats:sup>T<\/jats:sup> is affiliated with the genus <jats:italic>Methanocella<\/jats:italic>. The 16S rRNA gene sequences similarities within strains <jats:italic>Methanocella arvoryzae<\/jats:italic> MRE50<jats:sup>T<\/jats:sup>, <jats:italic>Methanocella paludicola<\/jats:italic> SANAE<jats:sup>T<\/jats:sup> and <jats:italic>Methanocella conradii<\/jats:italic> HZ254<jats:sup>T<\/jats:sup> were 93.7, 93.0 and 91.3\u200a%, respectively. In addition, the optical density of CWC-04<jats:sup>T<\/jats:sup> culture dropped abruptly upon entering the late-log growth phase, with virus-like particles (150\u2009nm in diameter) being observed on and around the cells. This observation suggests that strain CWC-04<jats:sup>T<\/jats:sup> harbours a lytic virus. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CWC-04<jats:sup>T<\/jats:sup> represents a novel species of a novel genus in the family <jats:italic>Methanocellaceae<\/jats:italic>, for which the name <jats:italic>Methanooceanicella nereidis<\/jats:italic> gen. nov., sp. nov. is proposed. The type strain is CWC-04<jats:sup>T<\/jats:sup> (=BCRC AR10050<jats:sup>T<\/jats:sup>=NBRC 113165<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006322","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T14:18:05Z","timestamp":1713449885000},"source":"Crossref","is-referenced-by-count":3,"title":["Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan"],"prefix":"10.1099","volume":"74","author":[{"given":"Wei-Ling","family":"Zhang","sequence":"first","affiliation":[{"name":"Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC"},{"name":"Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, 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