{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T06:03:14Z","timestamp":1783663394783,"version":"3.55.0"},"reference-count":26,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/100008388","name":"Danish Agricultural and Veterinary Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008388","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,13]]},"abstract":"<jats:p>A set of 25 strains belonging to clade V of <jats:italic>Mannheimia<\/jats:italic> mainly isolated from cattle was investigated and is proposed to represent <jats:italic>Mannheimia indoligenes<\/jats:italic> sp. nov. The species can be separated from the other validly published species of the genus by pheno- and genotype. Only indole separates <jats:italic>M. indoligenes<\/jats:italic> and <jats:italic>Mannheimia varigena<\/jats:italic> while two to seven characters separate <jats:italic>M. indoligenes<\/jats:italic> from other species of <jats:italic>Mannheimia<\/jats:italic>. Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98\u2013100\u200a% similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA\u2013DNA hybridization showed that the similarities between the suggested type strain M14.4<jats:sup>T<\/jats:sup> and the other strains of <jats:italic>M. indoligenes<\/jats:italic> were 62.9\u200a% or higher. The average nucleotide identity was 95.5\u200a% or higher between M14.4<jats:sup>T<\/jats:sup> and the other strains of the species. The <jats:italic>rpoB<\/jats:italic> gene sequence similarity was 95\u2013100\u200a% within <jats:italic>M. indoligenes<\/jats:italic>. MALDI-TOF allowed a clear separation from other <jats:italic>Mannheimia<\/jats:italic> species further supporting classification as a novel species and making it the diagnostic identification tool of choice for <jats:italic>M. indoligenes<\/jats:italic>. The type strain is M14.4<jats:sup>T<\/jats:sup> (=CCUG 77347<jats:sup>T<\/jats:sup>=DSM 116804<jats:sup>T<\/jats:sup>) isolated from a cattle tongue in Scotland.<\/jats:p>","DOI":"10.1099\/ijsem.0.006370","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T17:16:03Z","timestamp":1715620563000},"source":"Crossref","is-referenced-by-count":5,"title":["Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-4658","authenticated-orcid":false,"given":"Henrik","family":"Christensen","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kuhnert","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, 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