{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:56:48Z","timestamp":1775912208209,"version":"3.50.1"},"reference-count":23,"publisher":"Microbiology Society","issue":"8","funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["K23-AI135090"],"award-info":[{"award-number":["K23-AI135090"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["K01- AI173398"],"award-info":[{"award-number":["K01- AI173398"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,21]]},"abstract":"<jats:p>Four strains (MSK211, MSK294<jats:sup>T<\/jats:sup>, MSK312, MSK433) of a novel <jats:italic>Dolosigranulum<\/jats:italic> species were cultured from nasopharyngeal swabs collected from mother\u2013infant dyads in southern Botswana. These strains grew optimally on tryptic soy agar with 5% sheep blood solid medium and in fastidious bacteria broth. Colonies on tryptic soy agar with 5% sheep blood agar appeared grey or white with a flat, smooth surface and variable alpha haemolysis. Cells were Gram-positive, non-spore-forming, non-motile cocci that lacked catalase or oxidase activity. Major fatty acids were C<jats:sub>16\u200a:\u200a0<\/jats:sub> (palmitic acid), C<jats:sub>18\u200a:\u200a1\u2009<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic> (oleic acid), and C<jats:sub>18\u200a:\u200a0<\/jats:sub> (stearic acid). Analyses of 16S rRNA gene sequences identified these strains as belonging to the genus <jats:italic>Dolosigranulum<\/jats:italic> (family <jats:italic>Carnobacteriaceae<\/jats:italic>), which currently contains only a single validly published species (<jats:italic>Dolosigranulum pigrum<\/jats:italic>). Whole-genome sequencing revealed that the genomes of these strains are 1.98\u20132.07 Mbp in size and have a G+C content of 39.6\u201339.9\u2009mol%. Comparisons of these genomes to publicly available genomes of <jats:italic>D. pigrum<\/jats:italic> yielded average nucleotide identities and <jats:italic>in silico<\/jats:italic> DNA\u2013DNA hybridization values of 92.3\u201392.9% and 49.1\u201351.4%, respectively. These results indicate that these strains represent a novel species of <jats:italic>Dolosigranulum<\/jats:italic>, for which we propose the name <jats:italic>Dolosigranulum savutiense<\/jats:italic> sp. nov., with the type strain MSK294<jats:sup>T<\/jats:sup> (=DSM 117171<jats:sup>T<\/jats:sup>=JCM 36673<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006498","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:26:42Z","timestamp":1724279202000},"source":"Crossref","is-referenced-by-count":5,"title":["Dolosigranulum savutiense sp. nov., isolated from human upper respiratory samples collected in Botswana"],"prefix":"10.1099","volume":"74","author":[{"given":"Elena B.","family":"Popowitch","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sefelani C.","family":"Boiditswe","sequence":"additional","affiliation":[{"name":"Botswana-University of Pennsylvania Partnership, Gaborone, Botswana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Z.","family":"Patel","sequence":"additional","affiliation":[{"name":"School of Medicine, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhoanna N.","family":"Aquino","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Kuzey","family":"Sozat","sequence":"additional","affiliation":[{"name":"Duke University, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian J.","family":"Caiazzo","sequence":"additional","affiliation":[{"name":"Duke University, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-346X","authenticated-orcid":false,"given":"Antonio","family":"Maldonado-Barrag\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5079-9920","authenticated-orcid":false,"given":"Jillian H.","family":"Hurst","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew P.","family":"Steenhoff","sequence":"additional","affiliation":[{"name":"Global Health Center, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"},{"name":"School of Medicine, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana"},{"name":"Division of Pediatric Infectious Diseases, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"},{"name":"Botswana-University of Pennsylvania Partnership, Gaborone, Botswana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew S.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Botswana-University of Pennsylvania Partnership, Gaborone, Botswana"},{"name":"School of Medicine, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana"},{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1993.tb01602.x"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2415-13-31"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02520-05"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.1202-1203.2001"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01373-07"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102978"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00245-10"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.03.008"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1101\/2022.12.19.22283664"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000001607"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-018-1154-3"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01297-0"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"R14","article-title":"barrnap 0.9: rapid ribosomal RNA prediction","author":"Seemann","year":"2013"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001755"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00425-21"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006300"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200004000-00021"},{"key":"R22","article-title":"Catalase Test","year":"2010"},{"key":"R23","author":"Sasser","year":"2006"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.006498?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:26:49Z","timestamp":1724279209000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.006498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,21]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8,21]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.006498","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,21]]}}}