{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:57:15Z","timestamp":1790787435341,"version":"4.1.0"},"reference-count":68,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"National Science Foundation","award":["1736255"],"award-info":[{"award-number":["1736255"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,29]]},"abstract":"<jats:p>Four methane-oxidizing bacteria, designated as strains WSC-6<jats:sup>T<\/jats:sup>, WSC-7<jats:sup>T<\/jats:sup>, SURF-1<jats:sup>T<\/jats:sup>, and SURF-2<jats:sup>T<\/jats:sup>, were isolated from Saddle Mountain Creek in southwestern Oklahoma, USA, and the Sanford Underground Research Facility (SURF) in Lead, South Dakota, USA. The strains were Gram-negative, motile, short rods that possessed intracytoplasmic membranes characteristic of type I methanotrophs. All four strains were oxidase-negative and weakly catalase-positive. Colonies ranged from pale pink to orange in colour. Methane and methanol were the only compounds that could serve as carbon and energy sources for growth. Strains WSC-6<jats:sup>T<\/jats:sup> and WSC-7<jats:sup>T<\/jats:sup> grew optimally at lower temperatures (25 and 20\u2009\u00b0C, respectively) compared to strains SURF-1<jats:sup>T<\/jats:sup> and SURF-2<jats:sup>T<\/jats:sup> (40\u2009\u00b0C). Strains WSC-6<jats:sup>T<\/jats:sup> and SURF-2<jats:sup>T<\/jats:sup> were neutrophilic (optimal pH of 7.5 and 7.3, respectively), while strains WSC-7<jats:sup>T<\/jats:sup> and SURF-1<jats:sup>T<\/jats:sup> were slightly alkaliphilic, with an optimal pH of 8.8. The strains grew best in media amended with \u22640.5%\u2009NaCl. The major cellular fatty acids were C<jats:sub>14\u200a:\u200a0<\/jats:sub>, C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u2009<jats:italic>\u03c9<\/jats:italic>8<jats:italic>c<\/jats:italic>, C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u2009<jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>, and C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u2009<jats:italic>\u03c9<\/jats:italic>5<jats:italic>c<\/jats:italic>. The DNA G+C content ranged from 51.5 to 56.0\u2009mol%. Phylogenetic analyses indicated that the strains belonged to the genus <jats:italic>Methylomonas<\/jats:italic>, with each exhibiting 98.6\u201399.6% 16S rRNA gene sequence similarity to closely related strains. Genome-wide estimates of relatedness (84.5\u201388.4% average nucleotide identity, 85.8\u201392.4% average amino acid identity and 27.4\u201335.0% digital DNA\u2013DNA hybridization) fell below established thresholds for species delineation. Based on these combined results, we propose to classify these strains as representing novel species of the genus <jats:italic>Methylomonas<\/jats:italic>, for which the names <jats:italic>Methylomonas rivi<\/jats:italic> (type strain WSC-6<jats:sup>T<\/jats:sup>=ATCC TSD-251<jats:sup>T<\/jats:sup>=DSM 112293<jats:sup>T<\/jats:sup>), <jats:italic>Methylomonas rosea<\/jats:italic> (type strain WSC-7<jats:sup>T<\/jats:sup>=ATCC TSD-252<jats:sup>T<\/jats:sup>=DSM 112281<jats:sup>T<\/jats:sup>), <jats:italic>Methylomonas aurea<\/jats:italic> (type strain SURF-1<jats:sup>T<\/jats:sup>=ATCC TSD-253<jats:sup>T<\/jats:sup>=DSM 112282<jats:sup>T<\/jats:sup>), and <jats:italic>Methylomonas subterranea<\/jats:italic> (type strain SURF-2<jats:sup>T<\/jats:sup>=ATCC TSD-254<jats:sup>T<\/jats:sup>=DSM 112283<jats:sup>T<\/jats:sup>) are proposed.<\/jats:p>","DOI":"10.1099\/ijsem.0.006506","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T15:12:47Z","timestamp":1724944367000},"source":"Crossref","is-referenced-by-count":7,"title":["Methylomonas rivi sp. nov., Methylomonas rosea sp. nov., Methylomonas aurea sp. nov. and Methylomonas subterranea sp. nov., type I methane-oxidizing bacteria isolated from a freshwater creek and the deep terrestrial subsurface"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2325-3188","authenticated-orcid":false,"given":"Christopher A.","family":"Abin","sequence":"first","affiliation":[{"name":"Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, Oklahoma, USA"},{"name":"School of Biological Sciences, University of Oklahoma, Norman, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher 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