{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T05:09:52Z","timestamp":1733461792012,"version":"3.30.1"},"reference-count":49,"publisher":"Microbiology Society","issue":"12","funder":[{"name":"Ministry of Education, Taiwan, Republic of China under the ATU plan, the Central Geological Survey, MOEA","award":["99-5226904000-04-03"],"award-info":[{"award-number":["99-5226904000-04-03"]}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST103\/104\/105\/106\/107-3113-M-005-001"],"award-info":[{"award-number":["MOST103\/104\/105\/106\/107-3113-M-005-001"]}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST110-2320-B-039-058"],"award-info":[{"award-number":["MOST110-2320-B-039-058"]}]},{"DOI":"10.13039\/501100012544","name":"China Medical University, Taiwan","doi-asserted-by":"crossref","award":["CMU113-MF-99"],"award-info":[{"award-number":["CMU113-MF-99"]}],"id":[{"id":"10.13039\/501100012544","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100020719","name":"Sanming University","doi-asserted-by":"crossref","award":["20YG09"],"award-info":[{"award-number":["20YG09"]}],"id":[{"id":"10.13039\/100020719","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science and Technology Council","award":["NSTC 113-2320-B-039-016"],"award-info":[{"award-number":["NSTC 113-2320-B-039-016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,4]]},"abstract":"<jats:p>A hydrogenotrophic methanoarchaeon, designated strain FWC-SCC4<jats:sup>T<\/jats:sup>, was isolated from cold seep sediment of Four-Way Closure Ridge, offshore southwestern Taiwan. Strain FWC-SCC4<jats:sup>T<\/jats:sup>utilizes H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub> or formate, but not acetate, secondary alcohols, methylamines, methanol or ethanol for growth and methane production. Yeast extract is required for growth. The cell morphology is coccoid, with a diameter of 0.8\u20131.2\u2009\u00b5m, and the cell envelope is composed of S-layer protein with M<jats:sub>r<\/jats:sub> about 137.00\u2009kDa. Cells possess multiple flagella and usually occur singly. Strain FWC-SCC4<jats:sup>T<\/jats:sup> grows at a temperature range of 20\u201340 \u00b0C (optimum 37 \u00b0C) and a pH range of 5.4\u20137.2 (optimum 7.0). The NaCl range for growth is 0\u20130.86 M (optimum 0.09 M). The result of phylogenetic analysis of 16S rRNA gene sequences indicates that the most closely related species are <jats:italic>Methanoplanus limicola<\/jats:italic> M3<jats:sup>T<\/jats:sup> and <jats:italic>Methanoplanus endosymbiosus<\/jats:italic> MC1<jats:sup>T<\/jats:sup>, with similarities of 95.95 and 95.63%, respectively. The G+C content of the genomic DNA is 40.3\u2009mol%. The overall genome-relatedness indexes (OGRIs) and concatenated ribosomal protein (RBP) phylogenic analysis indicate that strain FWC-SCC4<jats:sup>T<\/jats:sup> is a novel lineage of <jats:italic>Methanomicrobiaceae<\/jats:italic>. In addition to strain FWC-SCC4<jats:sup>T<\/jats:sup>, the differences between <jats:italic>Methanomicrobium antiquum<\/jats:italic> MobH<jats:sup>T<\/jats:sup> and <jats:italic>Methanomicrobium mobile<\/jats:italic> BP<jats:sup>T<\/jats:sup> demonstrated by comparative analysis of genomic G+C content and phylogenetic analysis with non-type strain genomes are enough to support the establishment of a novel genus. In conclusion, strain FWC-SCC4<jats:sup>T<\/jats:sup> (BCRC AR10058<jats:sup>T<\/jats:sup>= NBRC 114595<jats:sup>T<\/jats:sup>) is proposed as the type strain of <jats:italic>Methanochimaera problematica<\/jats:italic> gen. nov., sp. nov., and <jats:italic>Methanoeremita antiquus<\/jats:italic> gen. nov., comb. nov. is proposed as the new name for <jats:italic>Methanomicrobium antiquum<\/jats:italic> MobH<jats:sup>T<\/jats:sup> (=DSM 21220<jats:sup>T<\/jats:sup>= NBRC 104160<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006593","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T11:16:22Z","timestamp":1733397382000},"source":"Crossref","is-referenced-by-count":0,"title":["Methanochimaera problematica gen. nov., sp. nov., a novel methanoarchaeon isolated from cold seep sediment and reclassification of Methanomicrobium antiquum as Methanoeremita antiquus gen. nov., comb. nov."],"prefix":"10.1099","volume":"74","author":[{"given":"Yang-Zhi","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Chin","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Ting","family":"You","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7653-8147","authenticated-orcid":false,"given":"Shu-Jung","family":"Lai","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, China Medical University, Taichung City, Taiwan, 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