{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:43:41Z","timestamp":1792691021503,"version":"4.5.0"},"reference-count":40,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:p>\n                    Isolates of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. were cultured from water sources at five different sites in central Iowa in the Midwestern United States and characterized by whole-genome sequencing. Isolates were helix-shaped and motile. Genome sequence analyses determined that the isolates could be clearly distinguished from other species described in the genus\n                    <jats:italic>Leptospir<\/jats:italic>\n                    a and included one species that belonged to the pathogen subclade P1, one species that belonged to the pathogen subclade P2 and three species that belonged to the saprophyte subclade S1. The names\n                    <jats:italic>Leptospira gorisiae<\/jats:italic>\n                    sp. nov. (type strain WS92.C1\n                    <jats:sup>T<\/jats:sup>\n                    =NVSL-WS92.C1\n                    <jats:sup>T<\/jats:sup>\n                    =KIT0303\n                    <jats:sup>T<\/jats:sup>\n                    ),\n                    <jats:italic>Leptospira cinconiae<\/jats:italic>\n                    sp. nov. (type strain WS58.C1\n                    <jats:sup>T<\/jats:sup>\n                    =NVSL-WS58.C1\n                    <jats:sup>T<\/jats:sup>\n                    =KIT0304\n                    <jats:sup>T<\/jats:sup>\n                    ),\n                    <jats:italic>Leptospira mgodei<\/jats:italic>\n                    sp. nov. (type strain WS4.C2\n                    <jats:sup>T<\/jats:sup>\n                    =NVSL.WS4.C2\n                    <jats:sup>T<\/jats:sup>\n                    =KIT0305\n                    <jats:sup>T<\/jats:sup>\n                    ),\n                    <jats:italic>Leptospira iowaensis<\/jats:italic>\n                    sp. nov. (type strain WS39.C2\n                    <jats:sup>T<\/jats:sup>\n                    =NVSL-WS39.C2\n                    <jats:sup>T<\/jats:sup>\n                    =KIT0306\n                    <jats:sup>T<\/jats:sup>\n                    ) and\n                    <jats:italic>Leptospira milleri<\/jats:italic>\n                    sp. nov. (type strain WS60.C2\n                    <jats:sup>T<\/jats:sup>\n                    =NVSL-WS60.C2\n                    <jats:sup>T<\/jats:sup>\n                    =KIT0307\n                    <jats:sup>T<\/jats:sup>\n                    ) are proposed.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.006595","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T11:15:38Z","timestamp":1736248538000},"source":"Crossref","is-referenced-by-count":25,"title":["Leptospira gorisiae sp. nov, L. cinconiae sp. nov, L. mgodei sp. nov, L. milleri sp. nov and L. iowaensis sp. nov: five new species isolated from water sources in the Midwestern United States"],"prefix":"10.1099","volume":"75","author":[{"given":"Camila","family":"Hamond","sequence":"first","affiliation":[{"name":"National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bienvenido","family":"Tibbs-Cortes","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G. V.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"LeCount","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie J.","family":"Putz","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy","family":"Anderson","sequence":"additional","affiliation":[{"name":"National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA, 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