{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:17:44Z","timestamp":1789132664943,"version":"build-2803163510"},"reference-count":32,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danmarks Frie Forskningsfond","award":["3127-00035B"],"award-info":[{"award-number":["3127-00035B"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,9]]},"abstract":"<jats:p>Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S <jats:italic>rrs<\/jats:italic>, internal transcribed spacer and 23S <jats:italic>rrl<\/jats:italic> elements, as well as a single-copy core-gene (<jats:italic>hsp65<\/jats:italic>, <jats:italic>rpoB+C<\/jats:italic>, <jats:italic>secA<\/jats:italic> and <jats:italic>tuf<\/jats:italic>) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and <jats:italic>in silico<\/jats:italic> DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are <jats:italic>Mycobacterium wendilense<\/jats:italic> sp. nov. (Mu0050<jats:sup>T<\/jats:sup>=ITM 501390<jats:sup>T<\/jats:sup>=CCUG 77525<jats:sup>T<\/jats:sup>), <jats:italic>Mycobacterium burgundiense<\/jats:italic> sp. nov. (Mu0053<jats:sup>T<\/jats:sup>=ITM 501391<jats:sup>T<\/jats:sup>=CCUG 77526<jats:sup>T<\/jats:sup>), <jats:italic>Mycobacterium kokjensenii<\/jats:italic> sp. nov. (Mu0083<jats:sup>T<\/jats:sup>=ITM 501392<jats:sup>T<\/jats:sup>=CCUG 77527<jats:sup>T<\/jats:sup>) and <jats:italic>Mycobacterium holstebronense<\/jats:italic> sp. nov. (Mu0102<jats:sup>T<\/jats:sup>=ITM 501393<jats:sup>T<\/jats:sup>=CCUG 77528<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006620","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T16:15:31Z","timestamp":1736266531000},"source":"Crossref","is-referenced-by-count":8,"title":["Four novel nontuberculous mycobacteria species: Mycobacterium wendilense sp. nov., Mycobacterium burgundiense sp. nov., Mycobacterium kokjensenii sp. nov. and Mycobacterium holstebronense sp. nov. revived from a historical Danish strain collection"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5214-3420","authenticated-orcid":false,"given":"Xenia Emilie Sinding","family":"Iversen","sequence":"first","affiliation":[{"name":"International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-7598","authenticated-orcid":false,"given":"Erik Michael","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6430-4586","authenticated-orcid":false,"given":"Dorte Bek","family":"Folkvardsen","sequence":"additional","affiliation":[{"name":"International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0797-1080","authenticated-orcid":false,"given":"Erik","family":"Svensson","sequence":"additional","affiliation":[{"name":"International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, 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