{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:08:17Z","timestamp":1788570497535,"version":"build-2803163510"},"reference-count":40,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"crossref","award":["21200092"],"award-info":[{"award-number":["21200092"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,21]]},"abstract":"<jats:p>A clinical isolate, R131, was isolated from the peritoneal swab of a patient who suffered from ruptured appendicitis with abscess and gangrene in Hong Kong in 2018. Cells are facultatively anaerobic, non-motile, Gram-positive coccobacilli. Colonies were small, grey, semi-translucent, low convex and alpha-haemolytic. The bacterium grew on blood agar but not on Brain Heart Infusion (BHI) and Mueller\u2013Hinton agars. It was negative for catalase, oxidase, indole and aesculin hydrolysis. The initial identification attempts via matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry and 16S rRNA gene sequencing yielded inconclusive results. The 16S rRNA gene analysis showed that R131 shared &gt;99% nucleotide identity with certain uncultured <jats:italic>Actinomycetales<\/jats:italic> bacteria. In this retrospective investigation, a complete genome of R131 was constructed, disclosing a DNA G+C content of 64%. Phylogenetic analysis showed that the bacterium was mostly related to <jats:italic>Scrofimicrobium canadense<\/jats:italic> WB03_NA08, which was first described in 2020. However, its 16S rRNA gene shared only 94.15% nucleotide identity with that of <jats:italic>S. canadense<\/jats:italic> WB03_NA08. Notably, the orthoANI between R131 and <jats:italic>S. canadense<\/jats:italic> WB03_NA08 was 67.81%. A pan-genome analysis encompassing R131 and 4 <jats:italic>Scrofimicrobium<\/jats:italic> genomes showed 986 core gene clusters shared with the <jats:italic>Scrofimicrobium<\/jats:italic> species, along with 601 cloud genes. The average nucleotide identity comparisons within the pan-genome analysis ranged from 59.78 to 62.51% between R131 and the other <jats:italic>Scrofimicrobium<\/jats:italic> species. Correspondingly, the dDDH values ranged from 19.20 to 22.30%, while the POCP values spanned from 57.48 to 60.94%. Therefore, a novel species, <jats:italic>Scrofimicrobium appendicitidis<\/jats:italic> sp. nov., is proposed. The type strain is R131<jats:sup>T<\/jats:sup> (=JCM 36615<jats:sup>T<\/jats:sup>=LMG 33627<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006633","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T17:18:11Z","timestamp":1737479891000},"source":"Crossref","is-referenced-by-count":1,"title":["Scrofimicrobium appendicitidis sp. nov., isolated from a patient with ruptured appendicitis"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5457-6000","authenticated-orcid":false,"given":"Hiu-Yin","family":"Lao","sequence":"first","affiliation":[{"name":"Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, Hong Kong, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette Y. 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