{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T16:13:42Z","timestamp":1784650422524,"version":"3.55.0"},"reference-count":52,"publisher":"Microbiology Society","issue":"3","funder":[{"name":"Natural Science Foundation of PR. 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Here, we isolated the marine methanogen <jats:italic>Methanococcoides cohabitans<\/jats:italic> sp. nov. strain LMO-2<jats:sup>T<\/jats:sup> from a long-term AOM enrichment culture from the Northern Gulf of Mexico. Strain LMO-2<jats:sup>T<\/jats:sup> is Gram-stain-negative, irregular 0.5\u20131 \u00b5m coccus without flagella. It utilizes a variety of methylated compounds including methanol, monomethylamine, dimethylamine and trimethylamine for growth and methanogenesis. However, it does not grow on formate, acetate, dimethyl sulphate, H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, betaine and choline. The optimal conditions for growth were observed within a temperature range of 30\u201335 \u00b0C, a pH range of 7.0\u20138.0 and a salinity range of 2\u20134% NaCl. Based on the similarity and phylogeny of the 16S rRNA gene and genomic sequence, strain LMO-2<jats:sup>T<\/jats:sup> is classified within the genus <jats:italic>Methanococcoides<\/jats:italic>. Among the isolated type strains of the genus, strain LMO-2<jats:sup>T<\/jats:sup> exhibited the highest 16S rRNA gene sequence identity with <jats:italic>Methanococcoides vulcani<\/jats:italic> SLH33<jats:sup>T<\/jats:sup> (99.4%). The digital DNA\u2013DNA hybridization and average nucleotide identity based on genome sequence showed that strain LMO-2<jats:sup>T<\/jats:sup> shared the highest similarity with <jats:italic>Methanococcoides orientis<\/jats:italic> LMO-1<jats:sup>T<\/jats:sup>, with values of 27.3% and 83.4%, respectively. In conclusion, we isolated a methylotrophic methanogen from an AOM culture, and the isolated strain LMO-2<jats:sup>T<\/jats:sup> represented a novel species of the genus <jats:italic>Methanococcoides<\/jats:italic>, for which the name <jats:italic>Methanococcoides cohabitans<\/jats:italic> sp. nov. is proposed. The type strain is LMO-2<jats:sup>T<\/jats:sup> (=CGMCC 1.18051<jats:sup>T<\/jats:sup>=KCTC 25774<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006685","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T16:16:37Z","timestamp":1741018597000},"source":"Crossref","is-referenced-by-count":3,"title":["Methanococcoides cohabitans sp. nov., a marine methylotrophic methanogen isolated from an anaerobic methane-oxidizing enrichment culture"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1522-5688","authenticated-orcid":false,"given":"Sihan","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Polar Ecosystem and Climate Change, Ministry of Education and School of Oceanography, Shanghai Jiao Tong University, Shanghai 200030, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewen","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of 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