{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:03:20Z","timestamp":1793142200633,"version":"4.5.0"},"reference-count":30,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,12]]},"abstract":"<jats:p>An actinomycete strain that produces a new antibiotic haneummycin, designated KM77-8<jats:sup>T<\/jats:sup>, was isolated from marine sediment collected from Tokyo Bay near Haneda Airport, Japan. This strain was phylogenetically related to <jats:italic>Streptomyces pilosus<\/jats:italic> NBRC 12807<jats:sup>T<\/jats:sup>, with the highest similarity (99.65%) based on 16S rRNA gene sequences. In contrast, genome-based comparisons between strain KM77-8<jats:sup>T<\/jats:sup> and <jats:italic>S. pilosus<\/jats:italic> JCM 4403<jats:sup>T<\/jats:sup> (=NBRC 12807<jats:sup>T<\/jats:sup>) revealed that average nucleotide identity (ANI) and digital DNA\u2013DNA hybridization (dDDH) values were 87.29% and 33.00\u200a%, respectively. Moreover, genome-based comparisons between strain KM77-8<jats:sup>T<\/jats:sup> and other known <jats:italic>Streptomyces<\/jats:italic> species, including close relatives (more than 98.7% similarities of 16S rRNA gene sequences), showed that their ANI and dDDH values were &lt;90%\u2009and &lt;70%, respectively. The DNA G+C content of strain KM77-8<jats:sup>T<\/jats:sup> was 71.34%. The whole-cell hydrolysates of strain KM77-8<jats:sup>T<\/jats:sup> contained <jats:sc>ll<\/jats:sc>-diaminopimelic acid, galactose, glucose, mannose and ribose. The major menaquinones (MKs) of this strain were MK-9(H<jats:sub>6<\/jats:sub>) and MK-9(H<jats:sub>8<\/jats:sub>), and the major fatty acids (&gt;10%) were anteiso-C<jats:sub>15\u200a:\u200a0<\/jats:sub>, iso-C<jats:sub>15\u200a:\u200a0<\/jats:sub>, C<jats:sub>16\u200a:\u200a0<\/jats:sub>, iso-C<jats:sub>16\u200a:\u200a0<\/jats:sub> and summed feature 3 (C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c97<\/jats:italic>c<jats:italic>\/<\/jats:italic>C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c96<\/jats:italic>c). Several phenotypic differences were observed between strain KM77-8<jats:sup>T<\/jats:sup> and <jats:italic>S. pilosus<\/jats:italic> NBRC 12807<jats:sup>T<\/jats:sup>. Furthermore, supernatant and methanol extract of not <jats:italic>S. pilosus<\/jats:italic> NBRC 12807<jats:sup>T<\/jats:sup> but strain KM77-8<jats:sup>T<\/jats:sup> displayed antibacterial activity against <jats:italic>Enterococcus faecium<\/jats:italic> and contained haneummycin. On the basis of the polyphasic data, we propose strain KM77-8<jats:sup>T<\/jats:sup> as a novel species of the genus <jats:italic>Streptomyces<\/jats:italic>, named <jats:italic>Streptomyces haneummycinicus<\/jats:italic> sp. nov. The type strain is KM77-8<jats:sup>T<\/jats:sup> (=NBRC 115995<jats:sup>T<\/jats:sup>=DSM 115857<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006777","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T16:16:16Z","timestamp":1747066576000},"source":"Crossref","is-referenced-by-count":1,"title":["Streptomyces haneummycinicus sp. nov., a new antibiotic-producing actinomycete isolated from marine sediment"],"prefix":"10.1099","volume":"75","author":[{"given":"Moeka","family":"Uemura","sequence":"first","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moriyuki","family":"Hamada","sequence":"additional","affiliation":[{"name":"Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, 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