{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:02:14Z","timestamp":1788904934153,"version":"build-2803163510"},"reference-count":53,"publisher":"Microbiology Society","issue":"6","funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["42477274"],"award-info":[{"award-number":["42477274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Special project for outstanding scientific and technological innovation talents of Fujian Academy of Agricultural Sciences","award":["YCZX202407"],"award-info":[{"award-number":["YCZX202407"]}]},{"name":"Science and technology special project of Fujian Province","award":["2023L3022"],"award-info":[{"award-number":["2023L3022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,6]]},"abstract":"<jats:p>Four pink-pigmented bacteria, designated as strains sgz302003<jats:sup>T<\/jats:sup>, sgz302004, sgz302005 and sgz302006, were isolated from a paddy soil enrichment. The cells were aerobic, Gram-stain-negative, motile and short rods. The ranges and optima of the temperature, NaCl concentration and pH for growth were 25\u201337\u2009\u00b0C (optimum, 30\u2009\u00b0C), 0\u20132% (w\/v) (optimum, 0\u20130.5%) and pH 5.0\u201310.0 (optimum, pH 6.5), respectively. Major fatty acids were summed feature 8 (C<jats:sub>18:1<\/jats:sub>\n                  <jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic> and\/or C<jats:sub>18:1<\/jats:sub>\n                  <jats:italic>\u03c9<\/jats:italic>6<jats:italic>c<\/jats:italic>). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains were affiliated with the genus <jats:italic>Methylobacterium<\/jats:italic> and were most closely related to <jats:italic>Methylobacterium isbiliense<\/jats:italic> AR24<jats:sup>T<\/jats:sup> (99.2% sequence similarity), <jats:italic>Methylobacterium nodulans<\/jats:italic> ORS 206<jats:sup>T<\/jats:sup> (97.7%) and <jats:italic>Methylobacterium currus<\/jats:italic> PR1016A<jats:sup>T<\/jats:sup> (97.4%). The methanol dehydrogenase <jats:italic>mxaF<\/jats:italic> gene was absent, but <jats:italic>xoxF<\/jats:italic> was present. Phylogenomic tree supported that these four strains formed a distinct clade within <jats:italic>Methylobacterium<\/jats:italic>. Q-10 was detected as the only respiratory quinone in the studied strains. The genomic DNA G+C content was 70.7\u201370.8\u200a%. The orthologous average nucleotide identity and <jats:italic>in silico<\/jats:italic> digital DNA\u2013DNA hybridization values between the four strains and <jats:italic>M. isbiliense<\/jats:italic> AR24<jats:sup>T<\/jats:sup> were 91.8\u201391.9%\u2009and 53.0\u201353.3%, respectively, while those with <jats:italic>M. nodulans<\/jats:italic> ORS 206<jats:sup>T<\/jats:sup> were 86.3\u201386.5%\u2009and 25.5%. Phylogenomic, physiological and chemotaxonomic analyses indicate that the four strains represent a novel species within the genus <jats:italic>Methylobacterium<\/jats:italic>, for which the name <jats:italic>Methylobacterium oryzisoli<\/jats:italic> sp. nov. is proposed, with strain sgz302003<jats:sup>T<\/jats:sup> (= MCCC 1K09384<jats:sup>T<\/jats:sup>=KCTC 8693<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.006805","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T14:15:33Z","timestamp":1749219333000},"source":"Crossref","is-referenced-by-count":3,"title":["Methylobacterium oryzisoli sp. nov., a novel methylotrophic methanol-utilizing bacterium isolated from the paddy soil"],"prefix":"10.1099","volume":"75","author":[{"given":"Lu","family":"Liu","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian 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