{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T08:27:59Z","timestamp":1780388879445,"version":"3.54.1"},"reference-count":32,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32325002 and 9235130"],"award-info":[{"award-number":["32325002 and 9235130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science","award":["CAAS-ASTIP-2021-BIOMA-01, CAAS-CSGLCA-202301, and CAAS-ZDRW202305"],"award-info":[{"award-number":["CAAS-ASTIP-2021-BIOMA-01, CAAS-CSGLCA-202301, and CAAS-ZDRW202305"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["42372354"],"award-info":[{"award-number":["42372354"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Sichuan Province Science and Technology Department","award":["2024NSFTD0020"],"award-info":[{"award-number":["2024NSFTD0020"]}]},{"name":"the Netherlands Ministry of Education, Culture and Science","award":["0.24.002.002"],"award-info":[{"award-number":["0.24.002.002"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["Y2024PTO3"],"award-info":[{"award-number":["Y2024PTO3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,10]]},"abstract":"<jats:p>An anaerobic, thermophilic methanogen, designated strain LWZ-6<jats:sup>T<\/jats:sup>, was isolated from the Shengli oil field, China. The cells of strain LWZ-6<jats:sup>T<\/jats:sup> were non-motile cocci, with a diameter of 0.5\u20131.0 \u00b5m, and formed aggregates. They reduced methanol and monomethylamine into methane, using H<jats:sub>2<\/jats:sub> as an electron donor. Dimethylamine, trimethylamine and methanethiol, H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, formate, acetate, pyruvate, lactate and glucose were not used as energy sources. Strain LWZ-6<jats:sup>T<\/jats:sup> required yeast extract, acetate or CO<jats:sub>2<\/jats:sub> as carbon sources. Strain LWZ-6<jats:sup>T<\/jats:sup> grew at 35\u201365 \u00b0C (optimum 55 \u00b0C), pH 5.0\u20138.0 (optimum 6.0\u20136.5) and 0\u201360 g l<jats:sup>\u22121<\/jats:sup> NaCl (optimum 9 g l<jats:sup>\u22121<\/jats:sup>). The genome was 1.54 Mbp with a G+C content of 54.42 mol%. Strain LWZ-6<jats:sup>T<\/jats:sup> shared 83.54% 16S rRNA gene sequence identity with <jats:italic>Infirmifilum lucidum<\/jats:italic> strain 3507LT<jats:sup>T<\/jats:sup> in the class <jats:italic>Thermoprotei<\/jats:italic>. Phylogenetic analysis based on the 16S rRNA gene, along with phylogenomic analysis, indicated that strain LWZ-6<jats:sup>T<\/jats:sup> belonged to the candidate class \u2018<jats:italic>Candidatus<\/jats:italic> Methanomethylicia\u2019, which lacks cultivated representatives. Based on these findings, a new species within a new genus, <jats:italic>Methanosuratincola petrocarbonis<\/jats:italic> gen. nov., sp. nov., is proposed for LWZ-6<jats:sup>T<\/jats:sup> (=CCAM 1872<jats:sup>T<\/jats:sup>=JCM 39528<jats:sup>T<\/jats:sup>). In addition, we propose the <jats:italic>Methanosuratincolia<\/jats:italic> class. nov. for candidate class \u2018<jats:italic>Ca<\/jats:italic>. Methanomethylicia\u2019 represented by <jats:italic>Methanosuratincolaceae<\/jats:italic> fam. nov. and <jats:italic>Methanosuratincolales<\/jats:italic> ord. nov. within the phylum <jats:italic>Thermoproteota<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/ijsem.0.006839","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T15:16:01Z","timestamp":1752160561000},"source":"Crossref","is-referenced-by-count":2,"title":["Methanosuratincola petrocarbonis gen. nov., sp. nov., a methyl-reducing methanogen isolated from Shengli oil field, and proposal of Methanosuratincolaceae fam. nov., Methanosuratincolales ord. nov. and Methanosuratincolia classis nov. in the phylum Thermoproteota"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0073-965X","authenticated-orcid":false,"given":"Kejia","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Development and Application of Rural Renewable Energy, Biogas 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