{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:50:18Z","timestamp":1787010618770,"version":"build-2736575974"},"reference-count":49,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/100017545","name":"Institute of Veterinary Bacteriology, University of Bern","doi-asserted-by":"crossref","award":["REF-660-50"],"award-info":[{"award-number":["REF-660-50"]}],"id":[{"id":"10.13039\/100017545","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:p>\n                    Nine Gram-positive, non-motile, facultatively anaerobic cocci, designated EM39E\u1d40, JEK85, 18KM676, 21M1142, 18KM445\n                    <jats:sup>T<\/jats:sup>\n                    , 18KM444, 18KM245, 18KM583 and 21KM1573, were isolated from diverse animal material, including horse and pig skin, bovine mastitis milk and feline urine from a urinary tract infection. Phylogenomic analysis based on amino-acid alignment obtained from translated whole-genome sequences; digital DNA\u2013DNA hybridization (dDDH); 16S rRNA,\n                    <jats:italic>dnaJ<\/jats:italic>\n                    ,\n                    <jats:italic>hsp60<\/jats:italic>\n                    ,\n                    <jats:italic>rpoB<\/jats:italic>\n                    and\n                    <jats:italic>sodA<\/jats:italic>\n                    gene comparison; and MALDI-TOF MS spectral profiles placed the strains within the\n                    <jats:italic>Macrococcus caseolyticus<\/jats:italic>\n                    clade. They were closely related to\n                    <jats:italic>Macrococcus bohemicus<\/jats:italic>\n                    CCM 7100\u1d40,\n                    <jats:italic>Macrococcus epidermidis<\/jats:italic>\n                    CCM 7099\u1d40,\n                    <jats:italic>Macrococcus goetzii<\/jats:italic>\n                    CCM 4927\u1d40 and\n                    <jats:italic>Macrococcus capreoli<\/jats:italic>\n                    DSM 113939\n                    <jats:sup>T<\/jats:sup>\n                    . The novel species exhibited 22.30% dDDH and 79.49% average nucleotide identity (ANI) to each other, &lt;24%\u2009dDDH and &lt;82%\u2009ANI to their closest relatives, values below established species delineation thresholds, confirming their classification as two distinct novel species. Chemotaxonomic analysis identified, in both species, diphosphatidylglycerol, phosphatidylglycerol, aminolipid, glycolipids and phosphoglycolipids, as the major polar lipids. Fatty acid profiling revealed C\n                    <jats:sub>14\u200a:\u200a0<\/jats:sub>\n                    , C\n                    <jats:sub>16\u200a:\u200a1<\/jats:sub>\n                    \u2009\u03c911c, C\n                    <jats:sub>16\u200a:\u200a0<\/jats:sub>\n                    and C\n                    <jats:sub>18\u200a:\u200a1<\/jats:sub>\n                    \u2009\u03c913c as predominant components, with antesio-C\n                    <jats:sub>15\u200a:\u200a0<\/jats:sub>\n                    in one species. The peptidoglycan type was A3\u03b1\n                    <jats:sc>l<\/jats:sc>\n                    -Lys\u2013Gly2. The novel species can be distinguished from their closest relatives by their ability to grow in 12.5% NaCl. EM39E\u1d40, JEK85, 18KM676 and 21M1142 can be distinguished by positive reaction to\n                    <jats:sc>d<\/jats:sc>\n                    -ribose, and 18KM445\n                    <jats:sup>T<\/jats:sup>\n                    , 18KM444, 18KM245, 18KM583 and 21KM1573 by the absence of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -glucosidase activity and acid production from methyl-\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -\n                    <jats:sc>d<\/jats:sc>\n                    -glucopyranoside and maltose. The two novel species can be differentiated from each other by\n                    <jats:sc>d<\/jats:sc>\n                    -ribose metabolism and methyl-\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -\n                    <jats:sc>d<\/jats:sc>\n                    -glucopyranoside hydrolysis. 18KM445\n                    <jats:sup>T<\/jats:sup>\n                    , 18KM444, 18KM245, 18KM583 and 21KM1573 formed a distinct cluster of strains, designated\n                    <jats:italic>Macrococcus equi<\/jats:italic>\n                    sp. nov., with strain 18KM445\u1d40 (=DSM 118744\u1d40; =CCOS 2124\u1d40; =CCM 9438\u1d40) as the proposed type strain. The second cluster, containing strains EM39E\u1d40, JEK85, 18KM676 and 21M1142, was designated\n                    <jats:italic>Macrococcus animalis<\/jats:italic>\n                    sp. nov., with strain EM39E\u1d40 (=DSM 118743\u1d40; =CCOS 2125\u1d40; =CCM 9439\u1d40) as the proposed type strain.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.006861","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:15:15Z","timestamp":1753892115000},"source":"Crossref","is-referenced-by-count":4,"title":["Macrococcus animalis sp. nov. and Macrococcus equi sp. nov., isolated from different animals\u2019 origins"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6050-5236","authenticated-orcid":false,"given":"Chahrazed","family":"Belhout","sequence":"first","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology & Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University 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