{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:24:54Z","timestamp":1759883094297,"version":"build-2065373602"},"reference-count":57,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:p>A polyphasic study was undertaken to characterize strain G32<jats:sup>T<\/jats:sup>, an actinomycete isolated from soil collected on Giresun Island (Black Sea, Turkey).\u202f16S rRNA phylogeny placed the strain within the genus <jats:italic>Nonomuraea<\/jats:italic>, but \u226499.1% similarity to its nearest type strains, <jats:italic>Nonomuraea turkmeniaca<\/jats:italic> DSM 43926<jats:sup>T<\/jats:sup>, <jats:italic>Nonomuraea aridisoli<\/jats:italic> KC333<jats:sup>T<\/jats:sup>, <jats:italic>Nonomuraea candida<\/jats:italic> NRRL B-24552<jats:sup>T<\/jats:sup>, <jats:italic>Nonomuraea polychroma<\/jats:italic> DSM 43925<jats:sup>T<\/jats:sup> and <jats:italic>Nonomuraea endophytica<\/jats:italic> DSM 45385<jats:sup>T<\/jats:sup>, suggested that it potentially represents a novel taxon.\u202fThe total genome size is about 12.66\u2009Mb, and the digital G+C content is 69.6 mol%. The high-quality draft genome shared \u226492.16%\u2009average nucleotide identity and \u226447.0% digital DNA\u2013DNA hybridization with the closest related type strains, corroborating species-level distinctness. Comparative genomics against four type strains, <jats:italic>N. turkmeniaca<\/jats:italic> DSM 43926<jats:sup>T<\/jats:sup>, <jats:italic>N. polychrom<\/jats:italic> DSM 43925<jats:sup>T<\/jats:sup>, <jats:italic>N. aridisoli<\/jats:italic> KC333<jats:sup>T<\/jats:sup> and <jats:italic>N. endophytica<\/jats:italic> DSM 45385<jats:sup>T<\/jats:sup>, revealed an open pangenome of 24,056 orthologous families, of which G32<jats:sup>T<\/jats:sup> contributed 2,785 singletons. Copy-number expansion of <jats:italic>aqpZ<\/jats:italic> with four paralogues and <jats:italic>efeB<\/jats:italic> with three paralogues, together with the sole genome-wide occurrence of ACC-deaminase and an additional <jats:italic>fhu<\/jats:italic>DBC siderophore transporter, highlights adaptations to periodic salinity, iron limitation and rhizosphere contact in island soil. Chemotaxonomic markers (<jats:italic>meso<\/jats:italic>-diaminopimelic, madurose, MK-9(H<jats:sub>4<\/jats:sub>), <jats:italic>iso<\/jats:italic>-C<jats:sub>16\u200a:\u200a0<\/jats:sub>, <jats:italic>iso<\/jats:italic>-C<jats:sub>16\u200a:\u200a0<\/jats:sub> 2OH and C<jats:sub>17\u200a:\u200a0<\/jats:sub> 10-<jats:italic>methyl<\/jats:italic>) of the strain were consistent with the genus <jats:italic>Nonomuraea<\/jats:italic>, but phenotypic traits further differentiated the isolate from its relatives. Based on the evidence presented here, strain G32<jats:sup>T<\/jats:sup> represents a novel species of the genus <jats:italic>Nonomuraea<\/jats:italic>, for which the name <jats:italic>Nonomuraea turcica<\/jats:italic> sp. nov. is proposed. The type strain of the species is G32<jats:sup>T<\/jats:sup> (=KCTC 49887<jats:sup>T<\/jats:sup>=CGMCC 4.7873<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.006938","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T15:26:25Z","timestamp":1759850785000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparative genomics and ecological insights into Nonomuraea turcica sp.\u202fnov., an actinomycete from Black Sea island soil"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8642-5872","authenticated-orcid":false,"given":"Hayrettin","family":"Saygin","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Sciences, Ondokuz Mayis University, 55139 Samsun, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-2-411"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1623"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004332"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-020-01941-9"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-021-01654-z"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004413"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-018-1097-6"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002954"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-019-01717-w"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001019"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-023-03427-w"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201300691"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.3390\/taxonomy5010005"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-020-01866-3"},{"key":"R15","first-page":"803","article-title":"New species of Actinomadura isolated from soils in Turkmenia and their antagonistic properties","volume":"26","author":"Galatenko","year":"1981","journal-title":"Antibiotiki"},{"key":"R16","first-page":"87","article-title":"New species, Actinomadura fulvescens sp. nov. and Actinomadura turkmeniaca sp. nov. and their antagonistic properties","volume":"27","author":"Terekhova","year":"1982","journal-title":"Antibiotiki"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.022558-0"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.3390\/md18040199"},{"key":"R19","doi-asserted-by":"crossref","first-page":"19126","DOI":"10.1073\/pnas.0906412106","article-title":"Shifting the genomic gold standard for the prokaryotic species definition","volume":"106","author":"Richter","year":"2019","journal-title":"Proc Natl Acad Sci USA"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"R21","first-page":"417","article-title":"Lichens of giresun district giresun province, Turkey","volume":"29","author":"Kinalio\u011flu","year":"2005","journal-title":"Turk J Bot"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.10.5005-5011.2002"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.17.2.103"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1021\/jf305210u"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-16-3-313"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.697-703.1991"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006421"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1003"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa374"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006300"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv150"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad313"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf334"},{"key":"R41","volume-title":"Classification, Identification and Descriptions of Genera and Species","volume":"2","author":"Waksman","year":"1961"},{"key":"R42","volume-title":"The Actinomycetes. 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