{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T13:53:33Z","timestamp":1770731613952,"version":"3.49.0"},"reference-count":90,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,9]]},"abstract":"<jats:p>\n                    Gram-positive, thermophilic, endospore-forming, yellow, rod-shaped bacteria, D401a\n                    <jats:sup>T<\/jats:sup>\n                    and D404, were isolated from soil samples of a hot spring in Dikili, Izmir. They can synthesize silver nanoparticles and biotechnologically important thermostable alpha-amylase, alpha-glucosidase and nitrate reductase, which were also confirmed by\n                    <jats:italic>in silico<\/jats:italic>\n                    analyses. 16S rRNA gene phylogenetic analysis revealed that D401a\n                    <jats:sup>T<\/jats:sup>\n                    was most closely related to D404 (99.9%) and had less than 98.9% similarity to\n                    <jats:italic>Anoxybacillus flavithermus<\/jats:italic>\n                    DSM 2641\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic>Anoxybacillus thermarum<\/jats:italic>\n                    DSM 7141\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic>Anoxybacillus mongoliensis<\/jats:italic>\n                    DSM 19169\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>Anoxybacillus ayderensis<\/jats:italic>\n                    from the genus\n                    <jats:italic>Anoxybacillus<\/jats:italic>\n                    . Their 2.7\u2009Mb whole-genome analyses indicated that strains D401a\n                    <jats:sup>T<\/jats:sup>\n                    and D404 represented a novel species within the genus\n                    <jats:italic>Anoxybacillus<\/jats:italic>\n                    , by displaying low average nucleotide identity and\n                    <jats:italic>in silico<\/jats:italic>\n                    DNA\u2013DNA hybridization values between\n                    <jats:italic>A. ayderensis<\/jats:italic>\n                    DSM 14988 (92.9%\u201350.5%),\n                    <jats:italic>A. ayderensis<\/jats:italic>\n                    DSM 10112\n                    <jats:sup>T<\/jats:sup>\n                    (92.4%\u201348.6%),\n                    <jats:italic>A. thermarum<\/jats:italic>\n                    DSM 17141\n                    <jats:sup>T<\/jats:sup>\n                    (92.6%\u201348.8%) and\n                    <jats:italic>Anoxybacillus gonensis<\/jats:italic>\n                    NCIMB 13933\n                    <jats:sup>T<\/jats:sup>\n                    (91.3%\u201343.8%) below 95% and 70%, respectively. The G+C content of genomic DNA was calculated to be 42.0\u2009mol%. The percentage of conserved protein values of two strains within the genus\n                    <jats:italic>Anoxybacillus<\/jats:italic>\n                    ranged from 89.3% to 85.0% and average amino acid identity values from 95.1% to 91.6%, indicating the overall genus-specific boundaries.\n                    <jats:italic>In silico<\/jats:italic>\n                    chemotaxonomic analysis revealed the presence of complete gene sets encoding iso-C15:0, iso-C17:0 and C16:0 fatty acids, menaquinone-7, polar lipids of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and meso-diaminopimelate-type peptidoglycan were identified. Their genomes encoded type III polyketide synthases that produce biotin. They harboured additional terpene\/carotenoid and terpene precursor biosynthetic clusters with secondary metabolites of carotenoids (C30), vitamin E and sesquiterpenes (C15). By morphological, physiological, phylogenetic and phylogenomic features, D401a\n                    <jats:sup>T<\/jats:sup>\n                    and D404 are assigned to be a new species of the genus\n                    <jats:italic>Anoxybacillus<\/jats:italic>\n                    , which the name\n                    <jats:italic>Anoxybacillus dikiliensis<\/jats:italic>\n                    sp. nov. is proposed (type strain D401a\n                    <jats:sup>T<\/jats:sup>\n                    =DSM 120222\n                    <jats:sup>T<\/jats:sup>\n                    =NCIMB 15614\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.006966","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T17:17:09Z","timestamp":1770657429000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic insight into Anoxybacillus dikiliensis sp. nov., a hot spring isolate from Dikili 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