{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T03:46:06Z","timestamp":1768621566428,"version":"3.49.0"},"reference-count":40,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"crossref","award":["191696\/V49"],"award-info":[{"award-number":["191696\/V49"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"crossref","award":["Living on Air 315129"],"award-info":[{"award-number":["Living on Air 315129"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"crossref","award":["346751"],"award-info":[{"award-number":["346751"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"crossref","award":["353750"],"award-info":[{"award-number":["353750"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,28]]},"abstract":"<jats:p>\n                    An aerobic methanotroph was isolated from a biofilm of coal mine Gruve 7 (Svalbard) and designated strain G7\n                    <jats:sup>T<\/jats:sup>\n                    . Cells of strain G7\n                    <jats:sup>T<\/jats:sup>\n                    were Gram-stain-negative, pink-pigmented and motile rods. Strain G7\n                    <jats:sup>T<\/jats:sup>\n                    could grow at pH 6.8 and at temperatures ranging from 4 to 21\u2009\u00b0C. The genome size was 4.00\u2009Mb with a (digital) DNA G+C content of 47.7 mol%. Strain G7\n                    <jats:sup>T<\/jats:sup>\n                    represents a member of the family\n                    <jats:italic>Methylomonadaceae<\/jats:italic>\n                    of the class\n                    <jats:italic>Gammaproteobacteria<\/jats:italic>\n                    . It displayed 94.6\u201399.7% 16S rRNA gene sequence similarity to the type strains of the genus\n                    <jats:italic>Methylobacter<\/jats:italic>\n                    . Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA\u2013DNA hybridization (dDDH) confirmed that strain G7\n                    <jats:sup>T<\/jats:sup>\n                    represents a novel species. It showed 16S rRNA gene identity of 99.7%, 91.8% ANI and 46% dDDH to the closest type strain,\n                    <jats:italic>Methylobacter svalbardensis<\/jats:italic>\n                    LS7-T4A\n                    <jats:sup>T<\/jats:sup>\n                    , with ANI and dDDH being much lower than the typically used 95 and 70% cutoffs, respectively, to delineate different species. For methane activation, strain G7\n                    <jats:sup>T<\/jats:sup>\n                    carries genes encoding particulate methane monooxygenase (pmoCAB). Also, genes of the methane utilization pathways, i.e. oxidation of methane to carbon dioxide and assimilation of methane-carbon to biomass, were encoded in the genome. Strikingly, compared to all other\n                    <jats:italic>Methylobacter<\/jats:italic>\n                    spp. strains, strain G7\n                    <jats:sup>T<\/jats:sup>\n                    did not have nitrogenase genes for nitrogen fixation. Strain G7\n                    <jats:sup>T<\/jats:sup>\n                    also possessed genes for ectoine production, which was not observed in the genomes of its closest relatives. Based on phenotypic, genetic and phylogenetic data, strain G7\n                    <jats:sup>T<\/jats:sup>\n                    represents a novel species within the genus\n                    <jats:italic>Methylobacter<\/jats:italic>\n                    for which the name\n                    <jats:italic>Methylobacter arcticus<\/jats:italic>\n                    sp. nov. is proposed, with strain G7\n                    <jats:sup>T<\/jats:sup>\n                    (DSM: 117899; LMG: 33632) as the type strain.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.006984","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T16:17:07Z","timestamp":1764346627000},"source":"Crossref","is-referenced-by-count":1,"title":["Methylobacter arcticus sp. nov. isolated from a coal mine biofilm in the high Arctic Svalbard"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5678-3361","authenticated-orcid":false,"given":"Antti 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