{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T17:21:10Z","timestamp":1764955270561,"version":"3.46.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,5]]},"abstract":"\n Four Gram-stain-positive bacterial strains, designated as YY-01\n T<\/jats:sup>\n , YY-02\n T<\/jats:sup>\n , YY-03\n T<\/jats:sup>\n and YY-04, were isolated from pit mud (YY-01\n T<\/jats:sup>\n , YY-02\n T<\/jats:sup>\n and YY-04) and daqu (YY-03\n T<\/jats:sup>\n ) samples collected at a winery in Shandong Province, China. Phylogenetic and genomic analyses, along with bioassays and antibiotic susceptibility testing, revealed that the four strains, YY-01\n T<\/jats:sup>\n , YY-02\n T<\/jats:sup>\n , YY-03\n T<\/jats:sup>\n and YY-04, represent three novel species within the genus\n Microbacterium<\/jats:italic>\n . Phylogenetic reconstruction based on 16S rRNA gene sequences revealed the closest relatives as follows: strain YY-01\n T<\/jats:sup>\n shares closest affinity with\n Microbacterium pseudoresistens<\/jats:italic>\n CC-005209\n T<\/jats:sup>\n (97.1% similarity), strains YY-02\n T<\/jats:sup>\n and YY-04 cluster with\n Microbacterium esteraromaticum<\/jats:italic>\n DSM 8609\n T<\/jats:sup>\n (98.3\u201398.4%\u2009similarity) and strain YY-03\n T<\/jats:sup>\n aligns with\n Microbacterium mitrae<\/jats:italic>\n M4-8\n T<\/jats:sup>\n (97.6% similarity). Genome phylogeny further resolved strain YY-01\n T<\/jats:sup>\n as a sister lineage to\n Microbacterium laevaniformans<\/jats:italic>\n DSM 20140\n T<\/jats:sup>\n , while strains YY-02\n T<\/jats:sup>\n , YY-03\n T<\/jats:sup>\n and YY-04 exhibited phylogenetic relationships congruent with the 16S rRNA topology. Genomic relatedness analysis demonstrated that strains YY-02\n T<\/jats:sup>\n and YY-04 share an OrthoANIu value of 99.9% and a digital DNA\u2013DNA hybridization (dDDH) value of 100%, confirming their classification as conspecific. In contrast, OrthoANIu and dDDH values between strains YY-01\n T<\/jats:sup>\n , YY-02\n T<\/jats:sup>\n and YY-03\n T<\/jats:sup>\n and other\n Microbacterium<\/jats:italic>\n species were \u226482.1\u2009and 28.3%, respectively, supporting their distinct species status. Chemotaxonomic analysis revealed distinct profiles for each strain. Strain YY-01\n T<\/jats:sup>\n was characterized by MK-13 menaquinones; major fatty acids anteiso-C\n 15\u200a:\u200a0<\/jats:sub>\n , anteiso-C\n 17\u200a:\u200a0<\/jats:sub>\n and iso-C\n 16\u200a:\u200a0<\/jats:sub>\n ; and the polar lipids diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG). Strain YY-02\n T<\/jats:sup>\n contained predominant menaquinones MK-11 and MK-13 and major fatty acids anteiso-C\n 17\u200a:\u200a0<\/jats:sub>\n , anteiso-C\n 15\u200a:\u200a0<\/jats:sub>\n and iso-C\n 16\u200a:\u200a0<\/jats:sub>\n , along with DPG and PG. Strain YY-03\n T<\/jats:sup>\n possessed major menaquinones MK-11 and MK-13; major fatty acids anteiso-C\n 15\u200a:\u200a0<\/jats:sub>\n , iso-C\n 15\u200a:\u200a0<\/jats:sub>\n and iso-C\n 16\u200a:\u200a0<\/jats:sub>\n ; and polar lipids DPG and PG. Integrative genomic, phenotypic and phylogenetic evidence supports the proposal of three novel species:\n Microbacterium luticellae<\/jats:italic>\n sp. nov. (type strain YY-01\n T<\/jats:sup>\n =GDMCC 1.5102\n T<\/jats:sup>\n =KCTC 59478\n T<\/jats:sup>\n ),\n Microbacterium alcoholitolerans<\/jats:italic>\n sp. nov. (type strain YY-02\n T<\/jats:sup>\n =GDMCC 1.5103\n T<\/jats:sup>\n =KCTC 59479\n T<\/jats:sup>\n ) and\n Microbacterium daqui<\/jats:italic>\n sp. nov. (type strain YY-03\n T<\/jats:sup>\n =GDMCC 1.5104\n T<\/jats:sup>\n =KCTC 59480\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijsem.0.006991","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T17:16:24Z","timestamp":1764954984000},"source":"Crossref","is-referenced-by-count":0,"title":["Description of Microbacterium luticellae sp. nov., Microbacterium alcoholitolerans sp. nov. and Microbacterium daqui sp. nov. isolated from baijiu pit mud and daqu"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8832-3413","authenticated-orcid":false,"given":"Mingkai","family":"Tian","sequence":"first","affiliation":[{"name":"School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Light Industry Science and Engineering, 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