{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:35:17Z","timestamp":1790094917249,"version":"4.0.1"},"reference-count":62,"publisher":"Microbiology Society","issue":"12","funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"crossref","award":["2021R1A2C3004015"],"award-info":[{"award-number":["2021R1A2C3004015"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Education, South Korea","award":["2020R1A6A1A06046235"],"award-info":[{"award-number":["2020R1A6A1A06046235"]}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"crossref","award":["RS-2023-00213601"],"award-info":[{"award-number":["RS-2023-00213601"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,19]]},"abstract":"<jats:p>\n                    A Gram-stain-negative, aerobic, motile, short rod-shaped methanotrophic bacterium, designated CM2\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from a reservoir pond in Korea. Cells of strain CM2\n                    <jats:sup>T<\/jats:sup>\n                    possessed a single polar flagellum, intracytoplasmic membrane stacks characteristic of type I methanotrophs, and contained electron-dense granules. The strain grew only on methane and methanol as carbon and energy sources. Growth occurred at 20\u201335\u2009\u00b0C (optimum, 30\u2009\u00b0C), pH 5.5\u20138.5 (optimum, 6.5) and with 0\u20130.5% (w\/w) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain CM2\n                    <jats:sup>T<\/jats:sup>\n                    within the genus\n                    <jats:italic>Methylomonas<\/jats:italic>\n                    , with sequence similarities to known species ranging from 94.5 to 98.3%. The major cellular fatty acids were C\n                    <jats:sub>16:1<\/jats:sub>\n                    and C\n                    <jats:sub>14:0<\/jats:sub>\n                    , and the predominant respiratory quinone was ubiquinone-8. The genome of strain CM2\n                    <jats:sup>T<\/jats:sup>\n                    is 5.06\u2009Mb in size with a G+C content of 56.8\u2009mol%. The digital DNA\u2013DNA hybridization, average nucleotide identity and average amino acid identity values between the genome of strain CM2\n                    <jats:sup>T<\/jats:sup>\n                    and those of\n                    <jats:italic>Methylomonas<\/jats:italic>\n                    species were 19.2\u201321.3\u200a%, 73.5\u201377.5\u200a% and 72.3\u201376.7\u200a%, respectively. Genome analysis identified genes encoding two distinct particulate forms and one soluble form of methane monooxygenase. Based on a polyphasic characterization, strain CM2\n                    <jats:sup>T<\/jats:sup>\n                    is considered to represent a novel species of\n                    <jats:italic>Methylomonas<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Methylomonas stagni<\/jats:italic>\n                    sp. nov. is proposed. The type strain is CM2\n                    <jats:sup>T<\/jats:sup>\n                    (KCTC 8468\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 36702\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007002","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T17:17:06Z","timestamp":1766164626000},"source":"Crossref","is-referenced-by-count":1,"title":["Methylomonas stagni sp. nov., a methanotroph of the genus Methylomonas, from a reservoir pond in Korea"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0464-4722","authenticated-orcid":false,"given":"Joo-Han","family":"Gwak","sequence":"first","affiliation":[{"name":"Present address: Department of Life Science, Hallym University, Chuncheon 24252, Republic of Korea"},{"name":"Department of Biological Sciences and Biotechnology, Chungbuk National University, 1 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