{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:59:33Z","timestamp":1782493173342,"version":"3.54.5"},"reference-count":61,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["1P20GM152333-01"],"award-info":[{"award-number":["1P20GM152333-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,16]]},"abstract":"\n Four strains of a Gram-stain-positive, coccoid, catalase-positive, non-motile bacterium were recovered from nasal lavage samples collected from children in Wisconsin during the Spring of 2008. These strains, designated RSM42\n T<\/jats:sup>\n , RSM292, RSM386 and RSM407, were subjected to a comprehensive biochemical and polyphasic taxonomic investigation. Despite the novel bacterium sharing 99.6% 16S rRNA gene sequence identity with\n Rothia mucilaginosa<\/jats:italic>\n 5762\/67\n T<\/jats:sup>\n , BLAST+ average nucleotide identity, MUMmer3 average nucleotide identity and digital DNA\u2013DNA hybridization values of 91.3%, 91.9% and 43.1%, respectively, were below the cut-off values routinely used for species demarcation. Consistent with these findings, phylogenetic and pangenomic comparisons indicated that RSM42\n T<\/jats:sup>\n , RSM292, RSM386 and RSM407 form a separate lineage within the genus\n Rothia<\/jats:italic>\n . Strain RSM42\n T<\/jats:sup>\n is further distinguished from\n R. mucilaginosa<\/jats:italic>\n 5762\/67\n T<\/jats:sup>\n by its unique ability among\n Rothia<\/jats:italic>\n species to use fructose-6-phosphate as a sole carbon source. RSM42\n T<\/jats:sup>\n also exhibits an enzyme activity profile consistent with\n R. mucilaginosa<\/jats:italic>\n , as it is positive for valine arylamidase and negative for C4 esterase, \u03b2-glucosidase, pyrazinamidase and trypsin, a combination not observed in other\n Rothia<\/jats:italic>\n species. The major fatty acids were anteiso-C\n 15:0<\/jats:sub>\n (44.2%) and iso-C\n 16:0<\/jats:sub>\n (14.4%), and the moderate fatty acids were anteiso-C\n 13:0<\/jats:sub>\n (2.3%), iso-C\n 14:0<\/jats:sub>\n (6.0%), C\n 14:0<\/jats:sub>\n (2.3%), iso-C\n 15:0<\/jats:sub>\n (5.9%), C\n 15:0<\/jats:sub>\n (1.9%), C\n 16:0<\/jats:sub>\n (9.3%) and anteiso-C\n 17:0<\/jats:sub>\n (9.5%). The major polar lipids were aminoglycolipid and diphosphatidylglycerol. Based on biochemical, phylogenetic, genotypic and chemotaxonomic criteria, these isolates represent a novel species within the genus\n Rothia<\/jats:italic>\n , closely related to\n R. mucilaginosa<\/jats:italic>\n , for which the name\n Rothia similimucilaginosa<\/jats:italic>\n sp. nov. is proposed. The type strain is RSM42\n T<\/jats:sup>\n (=ATCC TSD-447\n T<\/jats:sup>\n =DSM 118581\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijsem.0.007024","type":"journal-article","created":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:16:16Z","timestamp":1768565776000},"source":"Crossref","is-referenced-by-count":1,"title":["Rothia similimucilaginosa sp. nov., isolated from the human nasal cavity"],"prefix":"10.1099","volume":"76","author":[{"given":"Mercedes","family":"P\u00e9rez P\u00e9rez","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacobey","family":"King","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Oklahoma, Norman, OK 73019, 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