{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:34:38Z","timestamp":1773416078769,"version":"3.50.1"},"reference-count":40,"publisher":"Microbiology Society","issue":"3","funder":[{"name":"Open Project Fund of the Key Laboratory of Coal Environmental Pathogenicity and Prevention (Shanxi Medical University), Ministry of Education, China","award":["MEKLCEPP\/SXMU-202419"],"award-info":[{"award-number":["MEKLCEPP\/SXMU-202419"]}]},{"name":"Youth Fund for Enhancing Capability of Infectious Disease Surveillance and Prevention","award":["102393240020020000003"],"award-info":[{"award-number":["102393240020020000003"]}]},{"name":"Shanxi Eye Hospital","award":["B202201"],"award-info":[{"award-number":["B202201"]}]},{"name":"Young Top-notch Talent Project in the Medical and Health Field of the Sanjin Talents Program","award":["SJYC2024469"],"award-info":[{"award-number":["SJYC2024469"]}]},{"name":"Education Department of Shanxi Province","award":["2023L117"],"award-info":[{"award-number":["2023L117"]}]},{"DOI":"10.13039\/100017949","name":"Health Commission of Shanxi Province","doi-asserted-by":"crossref","award":["2023XG060"],"award-info":[{"award-number":["2023XG060"]}],"id":[{"id":"10.13039\/100017949","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100017949","name":"Health Commission of Shanxi Province","doi-asserted-by":"crossref","award":["2024149"],"award-info":[{"award-number":["2024149"]}],"id":[{"id":"10.13039\/100017949","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,13]]},"abstract":"<jats:p>\n                    Four novel bacterial strains, designated as G56\n                    <jats:sup>T<\/jats:sup>\n                    , G1551, G349\n                    <jats:sup>T<\/jats:sup>\n                    and G368, were isolated from\n                    <jats:italic>in situ<\/jats:italic>\n                    coal samples. All four strains were Gram-stain-positive, catalase-positive, oxidase-negative, non-motile and aerobic. Phylogenetic analysis based on 16S rRNA gene sequences revealed that G56\n                    <jats:sup>T<\/jats:sup>\n                    and G1551 represented the same species, and that G349\n                    <jats:sup>T<\/jats:sup>\n                    and G368 represented another species, with both species falling within the genus\n                    <jats:italic>Janibacter<\/jats:italic>\n                    . Strains G56\n                    <jats:sup>T<\/jats:sup>\n                    and G1551 exhibited the highest 16S rRNA gene sequence similarity to\n                    <jats:italic>Janibacter anophelis<\/jats:italic>\n                    CCUG 49715\n                    <jats:sup>T<\/jats:sup>\n                    (98.61%), whereas strains G349\n                    <jats:sup>T<\/jats:sup>\n                    and G368 exhibited the highest 16S rRNA gene sequence similarity to\n                    <jats:italic>Janibacter limosus<\/jats:italic>\n                    DSM 11140\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>Janibacter terrae<\/jats:italic>\n                    NBRC 107853\n                    <jats:sup>T<\/jats:sup>\n                    (99.03\u201399.10%). The average nucleotide identity and digital DNA\u2013DNA hybridization values between the proposed type strains (G56\n                    <jats:sup>T<\/jats:sup>\n                    and G349\n                    <jats:sup>T<\/jats:sup>\n                    ) and species of the genus\n                    <jats:italic>Janibacter<\/jats:italic>\n                    were 74.59\u201389.54%\u2009and 19.70\u201338.30%, respectively. The DNA G+C contents of G56\n                    <jats:sup>T<\/jats:sup>\n                    and G349\n                    <jats:sup>T<\/jats:sup>\n                    were 71.0 and 71.5\u2009mol%, respectively, and both strains contained only menaquinone MK-8(H\n                    <jats:sub>4<\/jats:sub>\n                    ) as the respiratory quinone. The major cellular fatty acids of strain G56\n                    <jats:sup>T<\/jats:sup>\n                    were C\n                    <jats:sub>16:0<\/jats:sub>\n                    10-methyl and\/or iso-C\n                    <jats:sub>17:1<\/jats:sub>\n                    <jats:italic>\u2009\u03c9<\/jats:italic>\n                    9\n                    <jats:italic>c<\/jats:italic>\n                    , iso-C\n                    <jats:sub>17:0<\/jats:sub>\n                    and iso-C\n                    <jats:sub>15:0<\/jats:sub>\n                    , whereas those of strain G349\n                    <jats:sup>T<\/jats:sup>\n                    were C\n                    <jats:sub>18:1<\/jats:sub>\n                    <jats:italic>\u2009\u03c9<\/jats:italic>\n                    9\n                    <jats:italic>c<\/jats:italic>\n                    , iso-C\n                    <jats:sub>16:0<\/jats:sub>\n                    and C\n                    <jats:sub>17:1<\/jats:sub>\n                    <jats:italic>\u2009\u03c9<\/jats:italic>\n                    8\n                    <jats:italic>c<\/jats:italic>\n                    . The polar lipid profiles of both strains comprised diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, and\n                    <jats:italic>meso<\/jats:italic>\n                    -diaminopimelic acid was identified as the diagnostic diamino acid in the cell wall. Based on phenotypic, phylogenetic and chemotaxonomic evidence, strains G56\n                    <jats:sup>T<\/jats:sup>\n                    and G349\n                    <jats:sup>T<\/jats:sup>\n                    were considered to represent two novel species of the genus\n                    <jats:italic>Janibacter<\/jats:italic>\n                    , for which the names\n                    <jats:italic>Janibacter zhaoxuefangae<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Janibacter zhangyanbonis<\/jats:italic>\n                    sp. nov. are proposed. The type strains are G56\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.5581\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 37886\n                    <jats:sup>T<\/jats:sup>\n                    ) and G349\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.5549\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 37887\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007097","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:17:12Z","timestamp":1773404232000},"source":"Crossref","is-referenced-by-count":0,"title":["Janibacter zhaoxuefangae sp. nov. and Janibacter zhangyanbonis sp. nov., two novel Actinobacteria isolated from in situ coal"],"prefix":"10.1099","volume":"76","author":[{"given":"Mei","family":"Zhang","sequence":"first","affiliation":[{"name":"Academy of Medical Sciences, Shanxi Medical University, Taiyuan 030001, PR China"},{"name":"Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Ministry of Education, Taiyuan, Shanxi 030001, PR China"},{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zixin","family":"Weng","sequence":"additional","affiliation":[{"name":"College of Modern Biomedical Industry, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China"},{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Kang","sequence":"additional","affiliation":[{"name":"Ophthalmic microbiology laboratory, Shanxi Eye Hospital, Taiyuan, Shanxi 030001, PR China"},{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caixin","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China"},{"name":"Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Ministry of Education, Taiyuan, Shanxi 030001, PR China"},{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taoyu","family":"Yan","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangyu","family":"Gao","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyao","family":"Xie","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Pu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Xu","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"},{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kui","family":"Dong","sequence":"additional","affiliation":[{"name":"Research Center for Reverse Microbial Etiology, Workstation of Academician, Shanxi Medical University, Taiyuan 030001, PR China"},{"name":"School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China"},{"name":"Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Ministry of Education, Taiyuan, Shanxi 030001, PR China"},{"name":"Ophthalmic microbiology laboratory, Shanxi Eye Hospital, Taiyuan, Shanxi 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-529"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1110"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006557"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-021-02745-w"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-3201-4"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1101\/gr.263566.120"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu077"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01940-w"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-141"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.038075-0"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1007\/BF01734359"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.2307\/2412116"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts565"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp077"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad359"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.531120"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64483-0"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1045"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1178"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-018-9824-9"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1139\/m96-128"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059527-0"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-27-2-104"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-52-1-77"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006855"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65293-0"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1080\/01483918208067640"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-100-2-221"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64768-0"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.29.319"},{"key":"R37","first-page":"277","article-title":"Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography","volume":"28","author":"Staneck","year":"1980","journal-title":"Appl Microbiol"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.002527-0"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-019-01247-x"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63167-0"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007097?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:17:15Z","timestamp":1773404235000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,13]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,13]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.007097","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,13]]}}}