{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:12:50Z","timestamp":1783401170672,"version":"3.54.6"},"reference-count":40,"publisher":"Microbiology Society","issue":"4","funder":[{"name":"Natural Sciences and Engineering Research Council of Canad"},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["JPMXD1420318865"],"award-info":[{"award-number":["JPMXD1420318865"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007802","name":"Institute for Fermentation, Osaka","doi-asserted-by":"crossref","award":["G-2022-1-007"],"award-info":[{"award-number":["G-2022-1-007"]}],"id":[{"id":"10.13039\/100007802","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"crossref","award":["23K28280"],"award-info":[{"award-number":["23K28280"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008957","name":"National Institute of Polar Research","doi-asserted-by":"crossref","award":["KP-404"],"award-info":[{"award-number":["KP-404"]}],"id":[{"id":"10.13039\/501100008957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,27]]},"abstract":"<jats:p>\n                    Two yeast strains, JCM 37756 and JCM 37757, were isolated from the sediments of Ward Hunt Lake, the northernmost lake in Canada, located at 83\u00b0 N on Ward Hunt Island, Nunavut. Molecular phylogenetic analysis based on the internal transcribed spacer (ITS) region and the D1\/D2 domain of the large subunit ribosomal DNA (rDNA) showed that these strains represent a novel species in the genus\n                    <jats:italic>Mrakia<\/jats:italic>\n                    . The two strains showed 100% sequence identity in the ITS region and only a single nucleotide difference in the D1\/D2 domain, confirming their conspecificity. Phylogenetic trees constructed using maximum likelihood, maximum parsimony and Bayesian inference consistently placed this new lineage as sister to the clade comprising\n                    <jats:italic>Mrakia fibulata<\/jats:italic>\n                    and the\n                    <jats:italic>Mrakia soli<\/jats:italic>\n                    clade. Although bootstrap support values were 59% (maximum-likelihood, ML) and 55% (maximum-parsimony, MP), the Bayesian posterior probability was 1.0, providing robust evidence for its taxonomic independence. Both strains are psychrophilic, growing at \u22123 \u00b0C in vitamin-free and amino acid-free media. We propose the name\n                    <jats:italic>Mrakia wardhuntensis<\/jats:italic>\n                    sp. nov., with JCM 37756 (=UAMH 12645) as the holotype. The MycoBank accession number is MB 858003.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007134","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T16:15:41Z","timestamp":1777306541000},"source":"Crossref","is-referenced-by-count":1,"title":["A novel psychrophilic yeast species, Mrakia wardhuntensis sp. nov., isolated from Ward Hunt Lake in the extreme High Arctic"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9375-7998","authenticated-orcid":false,"given":"Masaharu","family":"Tsuji","sequence":"first","affiliation":[{"name":"Department of Science of Technology Innovation, Nagaoka University of Technology, Nagaoka, Niigata, Japan"},{"name":"Present address: Depertment of Life Science, Toyo University, Asaka, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Girard","sequence":"additional","affiliation":[{"name":"D\u00e9partement de biochimie, de 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