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This strain is Gram-stain-negative, aerobic and non-sporulating, with rod-shaped cells and short filaments. Phylogenetic analyses based on 16S rRNA gene sequences (1,446\u2009bp) placed strain LT1-2-5\n                    <jats:sup>T<\/jats:sup>\n                    within the genus\n                    <jats:italic>Thermus<\/jats:italic>\n                    , showing highest similarities to\n                    <jats:italic>Thermus thalpophilus<\/jats:italic>\n                    SYSU G00506\n                    <jats:sup>T<\/jats:sup>\n                    (98.54%) and\n                    <jats:italic>Thermus brockianus<\/jats:italic>\n                    YS038\n                    <jats:sup>T<\/jats:sup>\n                    (98.34%). The strain exhibited optimal growth at 60\u201365\u2009\u00b0C, pH 7.0\u20138.0 and \u22642.0% (w\/v) sodium chloride (NaCl). Major cellular fatty acids (&gt;10%) were iso-C\n                    <jats:sub>17:0<\/jats:sub>\n                    , iso-C\n                    <jats:sub>15:0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>15:0<\/jats:sub>\n                    and anteiso-C\n                    <jats:sub>17:0<\/jats:sub>\n                    . The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. The major menaquinone was menaquinone-8. The genome size of strain LT1-2-5\n                    <jats:sup>T<\/jats:sup>\n                    was 2.41 Mbp, and the DNA G+C content was 66.8\u2009mol%. The average nucleotide identity and digital DNA\u2013DNA hybridization values between strain LT1-2-5\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>T. thalpophilus<\/jats:italic>\n                    SYSU G00506\n                    <jats:sup>T<\/jats:sup>\n                    were 91.46% and 42.9%, respectively, both of which are below the established species cut-off value. Polyphasic taxonomy, including phylogenetic, genomic, phenotypic and chemotaxonomic analyses, confirmed the status of strain LT1-2-5\n                    <jats:sup>T<\/jats:sup>\n                    as a novel species of\n                    <jats:italic>Thermus<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Thermus javaensis<\/jats:italic>\n                    sp. nov. is proposed. The type strain is LT1-2-5\n                    <jats:sup>T<\/jats:sup>\n                    (=UICC B-84\n                    <jats:sup>T<\/jats:sup>\n                    =CGMCC 1.61921\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 102310\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007136","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T15:17:08Z","timestamp":1777994228000},"source":"Crossref","is-referenced-by-count":0,"title":["Thermus javaensis sp. nov., a novel thermophilic bacterium isolated from litter of a geyser in Cisolok, West Java, Indonesia"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8008-7972","authenticated-orcid":false,"given":"Fitria","family":"Ningsih","sequence":"first","affiliation":[{"name":"Center of Excellence for Indigenous Biological Resources-Genome Studies, Faculty of 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