{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T17:07:02Z","timestamp":1779210422068,"version":"3.51.4"},"reference-count":45,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"crossref","award":["Ratchadaphiseksomphot Endowment Fund"],"award-info":[{"award-number":["Ratchadaphiseksomphot Endowment Fund"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,19]]},"abstract":"<jats:p>\n                    A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, designated JPR2-1\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from fermented fish (\n                    <jats:italic>pla-ra<\/jats:italic>\n                    ). Optimal growth was observed at 30\u201337\u2009\u00b0C, pH 7.0\u20137.5 and in the presence of 2\u20134% (w\/v) NaCl. It contained\n                    <jats:sc>l<\/jats:sc>\n                    -Orn-\n                    <jats:sc>d<\/jats:sc>\n                    -Asp in the cell wall peptidoglycan and MK-7 as the major menaquinone. The predominant fatty acids were iso-C\n                    <jats:sub>15:0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>15\u200a:0<\/jats:sub>\n                    and anteiso-C\n                    <jats:sub>17:0<\/jats:sub>\n                    . The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid. Strain JPR2-1\u1d40 belonged to the genus\n                    <jats:italic>Ornithinibacillus<\/jats:italic>\n                    and was based on 16S rRNA gene sequence similarity of 99.76, 99.76, 98.50, 98.18 and 98.08% with \u2018\n                    <jats:italic>Ornithinibacillus massiliensis<\/jats:italic>\n                    \u2019 Marseille-P3601\u1d40, \u2018\n                    <jats:italic>Ornithinibacillus hominis<\/jats:italic>\n                    \u2019 BX22\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic>Ornithinibacillus scapharcae<\/jats:italic>\n                    TW25\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic>Ornithinibacillus californiensis<\/jats:italic>\n                    MB-9\u1d40 and\n                    <jats:italic>Ornithinibacillus bavariensis<\/jats:italic>\n                    WSBC 24001\u1d40, respectively. The whole genome of strain JPR2-1\n                    <jats:sup>T<\/jats:sup>\n                    comprised 3,856,660 bp with 3,936 coding genes and a DNA G+C content of 37.3 mol%. The strain exhibited the average nucleotide identity values below 95% and lower than 70% of digital DNA\u2013DNA hybridization in comparison to\n                    <jats:italic>Ornithinibacillus<\/jats:italic>\n                    species, except \u2018\n                    <jats:italic>O. massiliensis<\/jats:italic>\n                    \u2019 Marseille-P3601\u1d40 and \u2018\n                    <jats:italic>O. hominis<\/jats:italic>\n                    \u2019 BX22\n                    <jats:sup>T<\/jats:sup>\n                    . This strain carried nine groups of intracellular hydrolytic genes (such as the rhomboid family and CAAX proteases) and three groups of extracellular proteolytic genes (the S8 family, the S9 family and zinc metallopeptidases). The highest specific activity of protease measured was 8.8\u2009U mg\n                    <jats:sup>\u22121<\/jats:sup>\n                    with the addition of 2.5% (w\/v) NaCl. The results of this polyphasic study indicate that strain JPR2-1\n                    <jats:sup>T<\/jats:sup>\n                    is a novel species in the genus\n                    <jats:italic>Ornithinibacillus<\/jats:italic>\n                    , and the name\n                    <jats:italic>Ornithinibacillus proteolyticus<\/jats:italic>\n                    sp. nov. is proposed. The type strain is JPR2-1\n                    <jats:sup>T<\/jats:sup>\n                    (=JCM 32622\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 33904\n                    <jats:sup>T<\/jats:sup>\n                    =TISTR 2557\n                    <jats:sup>T<\/jats:sup>\n                    ). In this study, \u2018\n                    <jats:italic>O. massiliensis<\/jats:italic>\n                    \u2019 and \u2018\n                    <jats:italic>O. hominis<\/jats:italic>\n                    \u2019 should be reclassified as a later heterotypic synonym of\n                    <jats:italic>O. proteolyticus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007164","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:16:42Z","timestamp":1779207402000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenomic insights, protease-related genes and protease of Ornithinibacillus proteolyticus sp. nov. isolated from fermented fish (Pla-ra)"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4739-4206","authenticated-orcid":false,"given":"Sirilak","family":"Namwong","sequence":"first","affiliation":[{"name":"Department of Food Innovation and Professional 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