{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:08:10Z","timestamp":1778864890562,"version":"3.51.4"},"reference-count":22,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"crossref","award":["26-21099S"],"award-info":[{"award-number":["26-21099S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:p>\n                    This study clarifies the taxonomic status of environmental\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    strains related to\n                    <jats:italic>Acinetobacter brisouii<\/jats:italic>\n                    , originally described based on a single strain in 2010. We analysed 15 strains, comprising\n                    <jats:italic>A. brisouii<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    =3) originating from Southeast Asia and 2 provisional taxa, Taxon 25A (\n                    <jats:italic>n<\/jats:italic>\n                    =6) and Taxon 25B (\n                    <jats:italic>n<\/jats:italic>\n                    =6), isolated from wetland habitats in Czechia. Their genome sizes ranged from 2.9 to 3.3\u2009Mb, with G+C contents of 41.2\u201341.8\u2009mol%. Core-genome phylogeny placed these strains into a distinct, deep-branching clade within the genus, structured into three strongly supported subclades corresponding to\n                    <jats:italic>A. brisouii<\/jats:italic>\n                    , Taxon 25A and Taxon 25B. Genomic average nucleotide identity and digital DNA\u2013DNA relatedness values were 95.9\u201399.7%\u2009and 66.1\u201398.3%, respectively, within the subclades\/taxa, 91.6\u201393.9%\u2009and 44.9\u201354.6% between the subclades\/taxa and &lt;77%\u2009and &lt;24%\u2009when the clade members were compared with other\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    species. The clade was further distinguished by metabolic and physiological traits and by whole-cell matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry profiles. All strains showed minimum inhibitory concentrations of colistin of 4\u201316\u2009mg l\u207b\u00b9, consistent with intrinsic resistance to polymyxins. Despite overall phenotypic similarity, the three taxa differed in several characteristics, including maximum growth temperature. We propose\n                    <jats:italic>Acinetobacter brisouisimilis<\/jats:italic>\n                    sp. nov. (type strain ANC 3789\u1d40=CCM 9537\u1d40=CCUG 78401\u1d40) and\n                    <jats:italic>Acinetobacter brisouioides<\/jats:italic>\n                    sp. nov. (type strain ANC 5584\u1d40=CCM 9536\u1d40=CCUG 78402\u1d40) for Taxon 25A and Taxon 25B, respectively, and provide an emended description of\n                    <jats:italic>A. brisouii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007166","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:16:29Z","timestamp":1778861789000},"source":"Crossref","is-referenced-by-count":0,"title":["Taxonomy of environmental strains related to Acinetobacter brisouii, with the proposal of Acinetobacter brisouisimilis sp. nov. and Acinetobacter brisouioides sp. nov., and an emended description of Acinetobacter brisouii"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2823-9043","authenticated-orcid":false,"given":"Lenka","family":"Radolfov\u00e1-K\u0159\u00ed\u017eov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Genetics, National Institute of Public Health, \u0160rob\u00e1rova 48, 100 00 Prague 10, Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"\u0160pan\u011blov\u00e1","sequence":"additional","affiliation":[{"name":"Czech National Collection of Type Cultures, National Institute of Public Health, \u0160rob\u00e1rova 48, 100 00 Prague 10, Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8658-0672","authenticated-orcid":false,"given":"Martina","family":"Maixnerov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genetics, National Institute of Public Health, \u0160rob\u00e1rova 48, 100 00 Prague 10, Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8677-9454","authenticated-orcid":false,"given":"Paul G.","family":"Higgins","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, Goldenfelstrasse 19\u201321, 50935 Cologne, and German Center for Infection Research (DZIF), Partner Site Bonn\u2013Cologne, Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8528-5321","authenticated-orcid":false,"given":"Martina","family":"Kyselkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague 4, Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0608-9598","authenticated-orcid":false,"given":"Alexandr","family":"Nemec","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, V \u00davalu 84, 150 06 Prague 5, Czechia"},{"name":"Laboratory of Bacterial Genetics, National Institute of Public Health, \u0160rob\u00e1rova 48, 100 00 Prague 10, Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","volume-title":"Bergey\u2019s Manual of Systematics of Archaea and Bacteria","author":"Nemec","year":"2022"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1110"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-009-0132-8"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034967-0"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evu225"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2024.126560"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2014.07.001"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001285"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2021.126217"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005642"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/jb.112.2.917-931.1972"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02550H"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2018.10.009"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.001230-0"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.11.006"},{"key":"R19","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing, 34th edn","year":"2024"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1186\/s42523-026-00568-3"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001903"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01261-13"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007166?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:16:30Z","timestamp":1778861790000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5,15]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.007166","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]}}}