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Strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    utilized methanol, trimethylamine, dimethylamine, monomethylamine and glycine betaine as substrates for growth and methane production, while strain CF\n                    <jats:sup>T<\/jats:sup>\n                    grew on H\n                    <jats:sub>2<\/jats:sub>\n                    \/CO\n                    <jats:sub>2<\/jats:sub>\n                    and formate. Cells of both strains were irregular cocci, with diameters of 0.5\u20131.3\u2009\u00b5m for strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    and 0.5\u20131.2\u2009\u00b5m for strain CF\n                    <jats:sup>T<\/jats:sup>\n                    . Using methanol as the substrate, strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    grew at 20\u201342\u2009\u00b0C (optimum 37\u2009\u00b0C), pH 5.5\u20138.0 (optimum 7.5) and 0\u20130.85 M NaCl (optimum 0.34 M NaCl). Strain CF\n                    <jats:sup>T<\/jats:sup>\n                    grew at 20\u201345\u2009\u00b0C (optimum 33\u201337\u2009\u00b0C), pH 6.0\u20138.5 (optimum 7.3) and 0\u20130.62 M NaCl (optimum 0.10\u20130.21 M NaCl) when growing on H\n                    <jats:sub>2<\/jats:sub>\n                    \/CO\n                    <jats:sub>2<\/jats:sub>\n                    . The genomic G+C contents are 44.4\u2009mol% for strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    and 33.4\u2009mol% for strain CF\n                    <jats:sup>T<\/jats:sup>\n                    . Phylogenetic analyses showed that strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    belongs to the genus\n                    <jats:italic>Methanococcoides<\/jats:italic>\n                    , sharing 85.20% average nucleotide identity (ANI) and 29.4% digital DNA\u2013DNA hybridization (dDDH) with its closest relative\n                    <jats:italic>Methanococcoides cohabitans<\/jats:italic>\n                    LMO-2\n                    <jats:sup>T<\/jats:sup>\n                    . Strain CF\n                    <jats:sup>T<\/jats:sup>\n                    is assigned to the genus\n                    <jats:italic>Methanococcus<\/jats:italic>\n                    , exhibiting 88.52% ANI and 35.3% dDDH with its closest relative\n                    <jats:italic>Methanococcus maripaludis<\/jats:italic>\n                    JJ\n                    <jats:sup>T<\/jats:sup>\n                    . Based on phenotypic and phylogenetic evidence, two novel species are proposed:\n                    <jats:italic>Methanococcoides mangrovi<\/jats:italic>\n                    sp. nov. (type strain FTZ1\n                    <jats:sup>T<\/jats:sup>\n                    =CCAM 1318\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 39395\n                    <jats:sup>T<\/jats:sup>\n                    ) and\n                    <jats:italic>Methanococcus mangrovi<\/jats:italic>\n                    sp. nov. (type strain CF\n                    <jats:sup>T<\/jats:sup>\n                    =CCAM 2191\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 39555\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007177","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T15:16:42Z","timestamp":1779463002000},"source":"Crossref","is-referenced-by-count":0,"title":["Methanococcoides mangrovi sp. nov. and Methanococcus mangrovi sp. nov., two novel methanogens isolated from mangrove sediments"],"prefix":"10.1099","volume":"76","author":[{"given":"Jinjie","family":"Zhou","sequence":"first","affiliation":[{"name":"Archaeal Biology Centre, Synthetic Biology Research Center, Shenzhen Key Laboratory of Marine Microbiome Engineering, Key Laboratory of Marine Microbiome Engineering of Guangdong Higher Education Institutes, Institute for Advanced Study, Shenzhen University, Shenzhen 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