{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:56:05Z","timestamp":1786636565763,"version":"build-2736575974"},"reference-count":31,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,13]]},"abstract":"<jats:p>\n                    Eleven novel Gram-stain-positive, catalase- and oxidase-negative coccal bacterial isolates recovered mostly from the wounds of patients located in three Canadian provinces were extensively studied. Phenotypic analysis and phylogenetic analysis based on both the 16S rRNA gene and whole-genome sequence place them in the family\n                    <jats:italic>Aerococcaceae<\/jats:italic>\n                    and differentiate them from genera in the closest families\n                    <jats:italic>Enterococcaceae<\/jats:italic>\n                    and\n                    <jats:italic>Carnobacteriaceae<\/jats:italic>\n                    in the order\n                    <jats:italic>Lactobacillales<\/jats:italic>\n                    . The G+C content of the isolates was on average 42.15\u2009mol% with genome sizes ranging from 1.87 to 2.04\u2009Mb. The average nucleotide identity and average amino acid identity values to other members of the family\n                    <jats:italic>Aerococcaceae<\/jats:italic>\n                    were below 69 and 62%,\u2009respectively, placing the isolates into a new genera. Phosphatidylserine is predicted to be synthesized based on the presence of the gene phosphatidylserine synthase in the annotated genome (E.C. 2.7.8.8).\n                    <jats:italic>In silico<\/jats:italic>\n                    analysis predicts the presence of\n                    <jats:sc>l<\/jats:sc>\n                    -lysine as the main diamino acid in the peptidoglycans. Major cellular fatty acids included C\n                    <jats:sub>16:0<\/jats:sub>\n                    , C\n                    <jats:sub>18:0<\/jats:sub>\n                    and C\n                    <jats:sub>18:1<\/jats:sub>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    9c. These 11 clinical strains were ultimately deemed to represent a novel genus and species, for which the name\n                    <jats:italic>Bernardella<\/jats:italic>\n                    gen. nov. and\n                    <jats:italic>Bernardella vulneris<\/jats:italic>\n                    sp. nov. is proposed and the type strain is NML130460\n                    <jats:sup>T<\/jats:sup>\n                    (=LMG 32739\n                    <jats:sup>T<\/jats:sup>\n                    =NCTC 14840\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007180","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:58:23Z","timestamp":1786633103000},"source":"Crossref","is-referenced-by-count":0,"title":["Description of Bernardella vulneris gen. nov., sp. nov., assignable to the family Aerococcaceae, isolated from human clinical samples"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1420-8323","authenticated-orcid":false,"given":"Anne-Marie","family":"Bernier","sequence":"first","affiliation":[{"name":"Department of Biology, Universit\u00e9 de Saint-Boniface, Winnipeg, MB, Canada"},{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6101-3655","authenticated-orcid":false,"given":"Dmytro","family":"Lyubashenko","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4319-8366","authenticated-orcid":false,"given":"Ana Luisa","family":"Pacheco","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-0847","authenticated-orcid":false,"given":"Mark","family":"Unger","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9789-2794","authenticated-orcid":false,"given":"Marc-Christian","family":"Domingo","sequence":"additional","affiliation":[{"name":"Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Institut national de sant\u00e9 publique du Qu\u00e9bec, Ste-Anne-de-Bellevue, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7999-8170","authenticated-orcid":false,"given":"Jennifer","family":"Tanner","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-021-01587-7"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006284"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004344"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003340"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1006\/anae.2000.0408"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn017"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040023"},{"key":"R11","article-title":"Draft genome sequence of trueperella bernardiae LCDC 89-0504T, isolated from a human blood culture","volume":"4","author":"Bernard","year":"2016","journal-title":"Genome Announc"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1105"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky425"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64483-0"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.7287\/peerj.preprints.1900v1"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003935"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz282"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv150"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz935"},{"key":"R25","volume-title":"Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria","year":"2016"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.11.006"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-021-01587-7"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.gpp3-0044-2018"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.1.83-89.1991"},{"key":"R30","volume-title":"Bruker Custom MSP and Library Creation","year":"2014"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001059"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007180?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:58:25Z","timestamp":1786633105000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,13]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.007180","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]}}}