{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T16:02:53Z","timestamp":1780329773574,"version":"3.54.1"},"reference-count":45,"publisher":"Microbiology Society","issue":"6","funder":[{"name":"Financial Fund of the Ministry of Agriculture and Rural Affairs of China","award":["NFZX2024"],"award-info":[{"award-number":["NFZX2024"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32460009"],"award-info":[{"award-number":["32460009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32460027"],"award-info":[{"award-number":["32460027"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"\n A Gram-stain-negative, motile, rod-shaped, alginate lyase-producing bacterium, designated HB246100\n T<\/jats:sup>\n , was isolated from coastal sand sample collected from Luhuitou Scenic Area, Sanya, China. The strain grew at 15\u201350 \u2103 (optimum, 35 \u2103), pH 6.0\u201311.0 (optimal, pH 8.0) and 0\u201310% (w\/v) NaCl (optimum, 3%). The predominant isoprenoid quinone was found to be menaquinone 7, and the major fatty acids were anteiso-C\n 15\u200a:\u200a0<\/jats:sub>\n , iso-C\n 15\u200a:\u200a0<\/jats:sub>\n , iso-C\n 14\u200a:\u200a0<\/jats:sub>\n and iso-C\n 16\u200a:\u200a0<\/jats:sub>\n . The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified lipid, three unidentified phospholipids and three unidentified glycolipids. The size of the genome was 4.29 Mbp, with a G+C content of 36.5 mol%. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB246100\n T<\/jats:sup>\n belonged to the genus\n Metabacillus<\/jats:italic>\n , and the closest phylogenetically related type species were\n Metabacillus crassostreae<\/jats:italic>\n JSM 100118\n T<\/jats:sup>\n ,\n Metabacillus schmidteae<\/jats:italic>\n Marseille-P9898\n T<\/jats:sup>\n ,\n Metabacillus halosaccharovorans<\/jats:italic>\n E33\n T<\/jats:sup>\n and\n Metabacillus litoralis<\/jats:ext-link>\n SW-211\n T<\/jats:sup>\n with the identities of 98.9, 98.1, 98.0 and 98.0%, respectively. The phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree based on core genome sequences indicated that the novel strain was most closely related to\n M. crassostreae<\/jats:italic>\n and\n M. litoralis<\/jats:italic>\n , with average nucleotide identity of 74.8\u201375.6%\u2009and digital DNA\u2013DNA hybridization value of 22.1\u201322.4%. Comprehensive phenotypic, phylogenetic and genomic characteristics demonstrate that strain HB246100\n T<\/jats:sup>\n is distinct from the species with validly published names in the genus\n Metabacillus<\/jats:italic>\n , suggesting that it represents a novel species of the genus\n Metabacillus<\/jats:italic>\n and proposing the name\n Metabacillus harenarius<\/jats:italic>\n sp. nov. The type strain is HB246100\n T<\/jats:sup>\n (=MCCC 1K09923\n T<\/jats:sup>\n =JCM 38130\n T<\/jats:sup>\n ). Furthermore, \u2018\n Bacillus weihaiensis<\/jats:italic>\n \u2019 Alg07, which only publicly disclosed its genome sequence, was emended and reclassified as\n M. harenarius<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1099\/ijsem.0.007185","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:15:36Z","timestamp":1780326936000},"source":"Crossref","is-referenced-by-count":0,"title":["Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of \u2018Bacillus weihaiensis\u2019"],"prefix":"10.1099","volume":"76","author":[{"given":"Rongting","family":"Liang","sequence":"first","affiliation":[{"name":"Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101\/Sanya 572024, PR China"},{"name":"Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, 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