{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:57:53Z","timestamp":1782917873327,"version":"3.54.5"},"reference-count":37,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"crossref","award":["JPMJFS2129"],"award-info":[{"award-number":["JPMJFS2129"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"crossref"}]},{"name":"New Energy and Industrial Technology Development Organization","award":["JPNP20011"],"award-info":[{"award-number":["JPNP20011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>\n                    A novel slow-growing bacterium, designated strain ag2_l2_5A\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from pine forest soil at Kagamiyama, Hiroshima, Japan, by using a gel microdroplet cultivation approach. The strain was characterized as a Gram-negative, non-motile bacterium with a short rod-shaped morphology. The strain formed small, circular, smooth and umbonate colonies after prolonged incubation on agar plates. Cells accumulated intracellular polyphosphate and produced membrane vesicles during the exponential growth phase. The strain grew optimally at 20\u201330\u2009\u00b0C\u2009and pH 6.0\u20137.5, utilizing various sugars, amino acids and organic acids as carbon sources. The genomic G+C content was 62.8%. The major fatty acids were iso-C\n                    <jats:sub>15\u200a:\u200a0<\/jats:sub>\n                    , C\n                    <jats:sub>16\u200a:\u200a1<\/jats:sub>\n                    \u2009\u03c97c, iso-C\n                    <jats:sub>16\u200a:\u200a0<\/jats:sub>\n                    , iso-C\n                    <jats:sub>17\u200a:\u200a0<\/jats:sub>\n                    and C\n                    <jats:sub>16\u200a:\u200a0<\/jats:sub>\n                    . Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain ag2_l2_5A\n                    <jats:sup>T<\/jats:sup>\n                    belongs to the class\n                    <jats:italic>Gemmatimonadetes<\/jats:italic>\n                    within the phylum\n                    <jats:italic>Gemmatimonadota<\/jats:italic>\n                    . Its closest relatives were\n                    <jats:italic>Gemmatimonas phototrophica<\/jats:italic>\n                    AP64\n                    <jats:sup>T<\/jats:sup>\n                    (91.3% gene sequence similarity),\n                    <jats:italic>Gemmatimonas aurantiaca<\/jats:italic>\n                    T-27\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>Pseudogemmatithrix spongiicola<\/jats:italic>\n                    318\n                    <jats:sup>T<\/jats:sup>\n                    . Based on polyphasic characterization, the strain ag2_l2_5A\n                    <jats:sup>T<\/jats:sup>\n                    represents a novel genus and species of the class\n                    <jats:italic>Gemmatimonadetes<\/jats:italic>\n                    , for which the name\n                    <jats:italic>Leucogemmata humicola<\/jats:italic>\n                    gen. nov., sp. nov. is proposed. The type strain is ag2_l2_5A\n                    <jats:sup>T<\/jats:sup>\n                    (=NBRC 117234\n                    <jats:sup>T<\/jats:sup>\n                    =DSM120521\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007204","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:39:53Z","timestamp":1782916793000},"source":"Crossref","is-referenced-by-count":0,"title":["Leucogemmata humicola gen. nov., sp. nov., an oligotrophic bacterium of the under-represented phylum Gemmatimonadota isolated from soil"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3486-9949","authenticated-orcid":false,"given":"Rikuta","family":"Suzuki","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-hiroshima, Hiroshima, 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