{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:18:46Z","timestamp":1783009126557,"version":"3.54.5"},"reference-count":58,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82360696"],"award-info":[{"award-number":["82360696"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Guangxi Natural Science Foundation","award":["2023GXNSFBA026240"],"award-info":[{"award-number":["2023GXNSFBA026240"]}]},{"name":"Burapha University through the National Research Council of Thailand","award":["Grant No. 46.2\/2562"],"award-info":[{"award-number":["Grant No. 46.2\/2562"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"\n In this study, three novel actinomycete strains, designated R6R-6\u1d40, R7R-8\u1d40 and R16R-3\u1d40, were isolated from the roots of\n Glycosmis pentaphylla<\/jats:italic>\n (Retz.) DC.,\n Aegle marmelos<\/jats:italic>\n (L.) Corr\u00eaa ex Roxb. and\n Citrus<\/jats:italic>\n \u00d7\n aurantiifolia<\/jats:italic>\n , respectively. The taxonomic status of these strains was evaluated using a polyphasic taxonomic approach. All three strains produced short spore chains (three to five spores) with cylindrical spores on aerial mycelia and exhibited chemotaxonomic characteristics consistent with members of the genus\n Nocardia<\/jats:italic>\n . These features included the presence of\n meso<\/jats:italic>\n -diaminopimelic acid and mycolic acids in the cell wall peptidoglycan, a polar lipid profile of type PII characterized by phosphatidylethanolamine, and cellular fatty acids (>1%) comprising C\n 14\u200a:\u200a0<\/jats:sub>\n , C\n 16\u200a:\u200a0<\/jats:sub>\n , C\n 17\u200a:\u200a0<\/jats:sub>\n , C\n 18\u200a:\u200a0<\/jats:sub>\n , C\n 18\u200a:\u200a0<\/jats:sub>\n 10-methyl, C\n 16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c911c, C\n 18\u200a:\u200a1<\/jats:sub>\n \u2009\u03c99c and C\n 16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c96c and\/or C\n 16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c97c. Based on 16S rRNA gene sequence analysis, strains R6R-6\u1d40 and R7R-8\u1d40 showed the highest sequence similarities of 98.9 and 99.1%, respectively, to\n Nocardia xestospongiae<\/jats:italic>\n ST01-07\u1d40, while strain R16R-3\u1d40 exhibited the highest similarity (98.5%) to\n Nocardia anaemiae<\/jats:italic>\n NBRC 100462\u1d40. Genome-based comparisons using digital DNA\u2013DNA hybridization and average nucleotide identity revealed that all three strains shared values below the established species delineation thresholds when compared with their closest phylogenetic relatives. Based on the combined phenotypic, phylogenetic and genomic evidence, strains R6R-6\u1d40, R7R-8\u1d40 and R16R-3\u1d40 represent three novel species of the genus\n Nocardia<\/jats:italic>\n , for which the names\n Nocardia glycosmidis<\/jats:italic>\n sp. nov. (type strain R6R-6\u1d40=TBRC 14548\n T<\/jats:sup>\n , =NBRC 115197\n T<\/jats:sup>\n ),\n Nocardia aegles<\/jats:italic>\n sp. nov. (type strain R7R-8\u1d40=TBRC 14549\n T<\/jats:sup>\n , =NBRC 115198\n T<\/jats:sup>\n ) and\n Nocardia citri<\/jats:italic>\n sp. nov. (type strain R16R-3\u1d40=TBRC 14550\n T<\/jats:sup>\n , =NBRC 115199\n T<\/jats:sup>\n ) are proposed.\n <\/jats:p>","DOI":"10.1099\/ijsem.0.007218","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T15:19:18Z","timestamp":1783005558000},"source":"Crossref","is-referenced-by-count":0,"title":["Nocardia glycosmidis, Nocardia aegles and Nocardia citri spp. nov., three novel endophytic actinomycetes isolated from the roots of Rutaceae plants"],"prefix":"10.1099","volume":"76","author":[{"given":"Paranee","family":"Sripreechasak","sequence":"first","affiliation":[{"name":"Department of Integrated Sciences, Faculty of Science, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achiraya","family":"Somphong","sequence":"additional","affiliation":[{"name":"Center of Excellence in Natural Products and Nanoparticles, Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Biochemistry and 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Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinpei","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory for Bioactive Molecules Research and Evaluation, Guangxi Key Laboratory of Composition-Activity Relationship of TCM, Pharmaceutical College, Guangxi Medical University, Nanning 530021, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1246-6700","authenticated-orcid":false,"given":"Wongsakorn","family":"Phongsopitanun","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Center of Excellence in Natural Products and Nanoparticles, Chulalongkorn University, Bangkok, 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