{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:17:59Z","timestamp":1783441079784,"version":"3.54.6"},"reference-count":39,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"the Japan Agency for Medical Research and Development\/Japan International Cooperation Agency","award":["JP24fk0108673"],"award-info":[{"award-number":["JP24fk0108673"]}]},{"name":"the Japan Agency for Medical Research and Development\/Japan International Cooperation Agency","award":["JP24wm0325054"],"award-info":[{"award-number":["JP24wm0325054"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP23K06554"],"award-info":[{"award-number":["JP23K06554"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP24K19189"],"award-info":[{"award-number":["JP24K19189"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP22K16382"],"award-info":[{"award-number":["JP22K16382"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:p>\n                    A rapidly growing acid-fast bacterium not consistent with existing species had previously been isolated from an immunocompetent patient with chronic and progressive pulmonary disease. Herein, we aimed to further characterize five clinically isolated strains based on genetic and phenotypic examinations and propose a novel species. The 16S rRNA gene sequence of the five strains had 99.6\u201399.7% similarity with that of\n                    <jats:italic>Mycobacterium mucogenicum<\/jats:italic>\n                    and clustered most closely to\n                    <jats:italic>M. mucogenicum<\/jats:italic>\n                    in a phylogenetic analysis based on single-copy marker genes. The average nucleotide identity values of the strains against\n                    <jats:italic>M. mucogenicum<\/jats:italic>\n                    and\n                    <jats:italic>Mycobacterium phocaicum<\/jats:italic>\n                    were \u226493.6% and \u226493.2%, respectively, indicating that they are separate species. The strains formed smooth colonies within 3\u20134 days and grew at temperatures between 24 and 42 \u00b0C, with optimal growth at 37 \u00b0C. The biochemical characteristics of the novel species were similar to those of\n                    <jats:italic>M. mucogenicum<\/jats:italic>\n                    and\n                    <jats:italic>M. phocaicum<\/jats:italic>\n                    . It predominantly contained C16:0 and C18:1 (n-9\n                    <jats:italic>cis<\/jats:italic>\n                    ) fatty acid and molecular species of mycolic acid, such as C77:2 and C79:2 of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -mycolic acids, C77:0 and C79:0 of keto-mycolic acids and C58:1 and C60:1 of dicarboxy-mycolic acids. Based on the genomic analyses, we propose a novel species, named\n                    <jats:italic>Mycobacterium toneyamaense,<\/jats:italic>\n                    with the type strain LRC2408\n                    <jats:sup>T<\/jats:sup>\n                    (=JCM 37957\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 59531\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007219","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:17:38Z","timestamp":1783437458000},"source":"Crossref","is-referenced-by-count":0,"title":["Mycobacterium toneyamaense sp. nov., a novel rapidly growing mycobacterium isolated from human patients"],"prefix":"10.1099","volume":"76","author":[{"given":"Takeshi","family":"Komine","sequence":"first","affiliation":[{"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Nakaya","sequence":"additional","affiliation":[{"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagatoshi","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Faculty of Contemporary Human Life Science, Tezukayama University, Nara, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoharu","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kida","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ato","sequence":"additional","affiliation":[{"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Mitarai","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2143-1160","authenticated-orcid":false,"given":"Hanako","family":"Fukano","sequence":"additional","affiliation":[{"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004922"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2014.10.003"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.tnmi7-0024-2016"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0331-1"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2022.10.013"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00067"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05626-y"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2024.116626"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00851-22"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.70078"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx713"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00476-24"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty149"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-021-1154-0"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-119"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006300"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv150"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx324"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16366-7"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"R35","volume-title":"Public Health Mycobacteriology: A Guide for the Level III Laboratory","author":"Kent","year":"1985"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002028"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.5772\/48301"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-10589-y"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27629-1"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007219?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:17:40Z","timestamp":1783437460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,7]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7,7]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.007219","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,7]]}}}