{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:24:51Z","timestamp":1788780291534,"version":"build-2803163510"},"reference-count":23,"publisher":"Microbiology Society","issue":"6","funder":[{"name":"Research Deputy of Shahrekord University","award":["grant number 141\/1402\/25"],"award-info":[{"award-number":["grant number 141\/1402\/25"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,30]]},"abstract":"<jats:p>\n                    <jats:italic>Periploca aphylla<\/jats:italic>\n                    witches\u2019 broom (PapWB) is a phytoplasma-associated disease first reported in south-western Iran in 2010. Recent observations showed that the disease is spreading to new areas. PapWB phytoplasmas, detected in symptomatic\n                    <jats:italic>P. aphylla<\/jats:italic>\n                    plants, exhibited identical nucleotide sequences in the 1.8\u2009kb PCR amplicon encompassing the 16S rRNA gene, the 16S-23S spacer region and the 5\u2032-end of the 23S rRNA gene. When compared with the reference strains of previously described \u2018\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Phytoplasma\u2019 species, the selected representative strain PapWB-S exhibited the highest 16S rRNA gene sequence identity (98.15%) with salt cedar witches\u2019 broom (SCWB1) phytoplasma, the reference strain of \u2018\n                    <jats:italic>Ca<\/jats:italic>\n                    . P. tamaricis\u2019. Moreover, the sequence identity values for the\n                    <jats:italic>tufB, rplV-rpsC<\/jats:italic>\n                    and\n                    <jats:italic>secY<\/jats:italic>\n                    genes of PapWB-S strain, when compared with those of reference strains of previously described \u2018\n                    <jats:italic>Ca<\/jats:italic>\n                    . Phytoplasma\u2019 species, were below the thresholds adopted for describing new species. Phylogenetic analyses confirmed the close relationship of the strain PapWB-S with SCWB1 followed by other \u2018\n                    <jats:italic>Ca<\/jats:italic>\n                    . Phytoplasma\u2019 species belonging to the ribosomal groups 16SrX, 16SrXX and 16SrXXXIII. Following the revised guidelines for describing \u2018\n                    <jats:italic>Ca<\/jats:italic>\n                    . Phytoplasma\u2019 species, \u2018\n                    <jats:italic>Ca<\/jats:italic>\n                    . P. periplocae\u2019 sp. nov., associated with PapWB disease, is proposed, and the 16S rRNA gene sequence (accessible at GenBank\/NCBI accession number OQ819043) is its nomenclatural type.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007225","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:17:35Z","timestamp":1782818255000},"source":"Crossref","is-referenced-by-count":1,"title":["\u2018Candidatus Phytoplasma periplocae\u2019 sp. nov. associated with Periploca aphylla witches\u2019 broom disease"],"prefix":"10.1099","volume":"76","author":[{"given":"Majid","family":"Siampour","sequence":"first","affiliation":[{"name":"Department of Plant Protection, College of Agriculture, Shahrekord University, Shahrekord, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keramatollah","family":"Izadpanah","sequence":"additional","affiliation":[{"name":"Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8866-0633","authenticated-orcid":false,"given":"Fabio","family":"Quaglino","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Sciences \u2013 Production, Landscape, Agroenergy, University of Milan, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Mehdi","family":"Faghihi","sequence":"additional","affiliation":[{"name":"Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Zarghan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assunta","family":"Bertaccini","sequence":"additional","affiliation":[{"name":"Alma Mater Studiorum, University of Bologna, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1123783"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2008.00472.x"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2016.05.031"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005353"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005977"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006502"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-1-174"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02854-0"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059774-0"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.3390\/biology11081119"},{"key":"R11","first-page":"1","volume-title":"Phytoplasmas: Plant Pathogenic Bacteria \u2013 1. 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