{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:11:56Z","timestamp":1785859916508,"version":"3.56.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,22]]},"abstract":"\n Five crocodilian species were investigated for the presence of\n Pasteurellaceae<\/jats:italic>\n . A total of 27 pharyngeal isolates were obtained and characterized by MALDI-TOF MS and preliminary phenotypic tests to enable selection of representative strains for further genotypic characterization. Twelve isolates were selected for sequencing followed by phylogenetic analyses, digital DNA\u2013DNA hybridization (dDDH) and calculation of average nucleotide identity (ANI), in addition to comparative analyses of partial\n rpoB<\/jats:italic>\n , 16S rRNA gene sequences and further phenotypic characterization. The phenotypic and genotypic comparisons grouped the strains into three clades (clade 1:\n n<\/jats:italic>\n =4; clade 2:\n n<\/jats:italic>\n =3; clade 3:\n n<\/jats:italic>\n =5). The clade 3 strains had a 16S rRNA gene sequence similarity ranging from 94.8 to 96.5%, a dDDH value of \u226448.7% and an ANI value of \u226492.6%\u2009to the closest relative,\n Testudinibacter aquarius<\/jats:italic>\n ELNT2x\n T<\/jats:sup>\n , and could be distinguished from all current\n Pasteurellaceae<\/jats:italic>\n species by a combination of phenotypic traits. Our results indicate that the clade 3 strains should be classified as representatives of a new\n Testudinibacter<\/jats:italic>\n species for which we suggest\n Testudinibacter crocodili<\/jats:italic>\n sp. nov. The type strain of\n T. crocodili<\/jats:italic>\n is Cuba42\n T<\/jats:sup>\n (=CCUG 77853\n T<\/jats:sup>\n =DSM 118260\n T<\/jats:sup>\n ) and was isolated in Denmark in 2018 from the pharynx of a captive Cuban crocodile (\n Crocodylus rhombifer<\/jats:italic>\n ).\n <\/jats:p>","DOI":"10.1099\/ijsem.0.007236","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T12:15:39Z","timestamp":1784895339000},"source":"Crossref","is-referenced-by-count":0,"title":["Testudinibacter crocodili sp. nov., a new member of the family Pasteurellaceae isolated from crocodilians"],"prefix":"10.1099","volume":"76","author":[{"given":"Troels","family":"Ronco","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8748-3554","authenticated-orcid":false,"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederik Stig","family":"Scharling","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil","family":"Lund","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4030-0019","authenticated-orcid":false,"given":"Anders Miki","family":"Bojesen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870 Frederiksberg C, 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