{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:58:45Z","timestamp":1785499125443,"version":"3.56.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["ZR2024QC137"],"award-info":[{"award-number":["ZR2024QC137"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31900091"],"award-info":[{"award-number":["31900091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["ZR2025MS462"],"award-info":[{"award-number":["ZR2025MS462"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,22]]},"abstract":"<jats:p>\n                    Two Gram-stain-negative, non-motile and oval-shaped bacterial strains, designated 2E107\n                    <jats:sup>T<\/jats:sup>\n                    and 2E233\n                    <jats:sup>T<\/jats:sup>\n                    , were isolated from the brown alga\n                    <jats:italic>Saccharina japonica<\/jats:italic>\n                    collected in Rongcheng, Weihai, China (122\u00b0 33\u2032 E 37\u00b0 15\u2032 N). The two strains shared 98.0% 16S rRNA gene sequence similarity. Both strains grew at 4\u201337\u2009\u00b0C, with optimum growth at 35\u2009\u00b0C, and tolerated 0.5\u201310% (\n                    <jats:italic>w\/v<\/jats:italic>\n                    ) NaCl. Strain 2E107\n                    <jats:sup>T<\/jats:sup>\n                    grew at pH 6.5\u20139.5, with optimum growth at pH 7.0 and 1.5% (\n                    <jats:italic>w\/v<\/jats:italic>\n                    ) NaCl, whereas strain 2E233\n                    <jats:sup>T<\/jats:sup>\n                    grew at pH 5.5\u20139.0, with optimum growth at pH 6.5 and 4% (\n                    <jats:italic>w\/v<\/jats:italic>\n                    ) NaCl. Strain 2E107\n                    <jats:sup>T<\/jats:sup>\n                    produced melanin, whereas strain 2E233\n                    <jats:sup>T<\/jats:sup>\n                    was non-pigmented. The genomic DNA G+C contents of strains 2E107\n                    <jats:sup>T<\/jats:sup>\n                    and 2E233\n                    <jats:sup>T<\/jats:sup>\n                    were 62.8 and 60.5 mol%, respectively. In both strains, summed feature 8 (C\n                    <jats:sub>18\u200a:\u200a1<\/jats:sub>\n                    <jats:italic>\u2009\u03c97<\/jats:italic>\n                    c and\/or C\n                    <jats:sub>18\u200a:\u200a1<\/jats:sub>\n                    <jats:italic>\u2009\u03c96<\/jats:italic>\n                    c) was the predominant cellular fatty acid, and ubiquinone-10 was the sole respiratory quinone. The major polar lipids of strain 2E107\n                    <jats:sup>T<\/jats:sup>\n                    were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid, whereas those of strain 2E233\n                    <jats:sup>T<\/jats:sup>\n                    were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The average nucleotide identity and average amino acid identity values between strain 2E107\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>Pacificoceanicola onchidii<\/jats:italic>\n                    XY-301\n                    <jats:sup>T<\/jats:sup>\n                    were 78.5 and 81.8%, respectively, whereas the corresponding values between strain 2E233\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>P. onchidii<\/jats:italic>\n                    XY-301\n                    <jats:sup>T<\/jats:sup>\n                    were 76.4 and 78.6%, respectively. On the basis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic evidence, strains 2E107\n                    <jats:sup>T<\/jats:sup>\n                    and 2E233\n                    <jats:sup>T<\/jats:sup>\n                    are considered to represent two novel species of the genus\n                    <jats:italic>Pacificoceanicola<\/jats:italic>\n                    , for which the names\n                    <jats:italic>Pacificoceanicola niger<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Pacificoceanicola algae<\/jats:italic>\n                    sp. nov. are proposed, respectively. The type strains are 2E107\n                    <jats:sup>T<\/jats:sup>\n                    (=MCCC 1H01529\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 8819\n                    <jats:sup>T<\/jats:sup>\n                    =SDUM152014\n                    <jats:sup>T<\/jats:sup>\n                    ) and 2E233\n                    <jats:sup>T<\/jats:sup>\n                    (=MCCC 1H01530\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 8818\n                    <jats:sup>T<\/jats:sup>\n                    =SDUM152015\n                    <jats:sup>T<\/jats:sup>\n                    ), respectively. Furthermore, on the basis of phylogenomic and comparative taxonomic evidence,\n                    <jats:italic>Tropicibacter oceani<\/jats:italic>\n                    Zhou\n                    <jats:italic>et al<\/jats:italic>\n                    . 2025 is proposed to be reclassified as\n                    <jats:italic>Pacificoceanicola oceani<\/jats:italic>\n                    comb. nov.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007251","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:16:17Z","timestamp":1785496577000},"source":"Crossref","is-referenced-by-count":0,"title":["Pacificoceanicola niger sp. nov. and Pacificoceanicola algae sp. nov., isolated from the brown alga Saccharina japonica, with the reclassification of Tropicibacter oceani as Pacificoceanicola oceani comb. nov."],"prefix":"10.1099","volume":"76","author":[{"given":"Lu","family":"Wang","sequence":"first","affiliation":[{"name":"Joint Science College, Shandong University, Weihai 264209, PR China"},{"name":"Marine College, Shandong University, Weihai 264209, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglin","family":"Sun","sequence":"additional","affiliation":[{"name":"Marine College, Shandong University, Weihai 264209, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Rongcheng Campus, Harbin University of Science and Technology, Weihai 264300, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"China-ASEAN Belt and Road Joint Laboratory on Marineculture Technology, Xiamen University, Xiamen 361102, PR China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, PR China"},{"name":"State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, 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