{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:29Z","timestamp":1792786169642,"version":"4.5.0"},"reference-count":57,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PNRA","award":["PNRA16_00274"],"award-info":[{"award-number":["PNRA16_00274"]}],"id":[{"id":"https:\/\/ror.org\/01b7car24","id-type":"ROR","asserted-by":"crossref"}]},{"name":"PNRA","award":["PNRA18_00232"],"award-info":[{"award-number":["PNRA18_00232"]}],"id":[{"id":"https:\/\/ror.org\/01b7car24","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Commission - NextGenerationEU","award":["NBFC n. CN_00000033"],"award-info":[{"award-number":["NBFC n. CN_00000033"]}],"id":[{"id":"https:\/\/ror.org\/00k4n6c32","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,17]]},"abstract":"<jats:p>\n                    A novel psychrophilic member of the phylum\n                    <jats:italic>Actinomycetota<\/jats:italic>\n                    , designated strain CPHL_1\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from the shoreline of Cape Hallett, Antarctica. Cells were Gram-positive, aerobic, non-motile, non-spore-forming rods (0.7\u20131.0\u2009\u00b5m in length) with orange to yellow pigmentation. Optimal growth occurred at 15\u2009\u00b0C, pH 8.0, with NaCl tolerance up to 3% (w\/v). Strain CPHL_1\n                    <jats:sup>T<\/jats:sup>\n                    exhibited cytosolic cold-active \u03b2-galactosidase activity, with an optimum at 15\u2009\u00b0C and pH 7.0 and formed non-adherent biofilms. Phylogenetic analysis based on 16S rRNA gene sequence placed the strain within the genus\n                    <jats:italic>Rhodoglobus<\/jats:italic>\n                    , showing high similarity to\n                    <jats:italic>Rhodoglobus vestalii<\/jats:italic>\n                    LV3\n                    <jats:sup>T<\/jats:sup>\n                    (99.4%) and\n                    <jats:italic>Rhodoglobus aureus<\/jats:italic>\n                    CMS 81y\n                    <jats:sup>T<\/jats:sup>\n                    (97.6%). Chemotaxonomic characteristics, including the predominance of anteiso-C\n                    <jats:sub>15\u200a:\u200a0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>17\u200a:\u200a0<\/jats:sub>\n                    and iso-C\n                    <jats:sub>16\u200a:\u200a0<\/jats:sub>\n                    fatty acids, the presence of MK-11, MK-10 and MK-12 as the major respiratory quinones, and genomic G+C content of 60%, further supported its assignment to the genus\n                    <jats:italic>Rhodoglobus<\/jats:italic>\n                    . Whole-genome comparisons revealed clear taxonomic distinction, with strain CPHL_1\n                    <jats:sup>T<\/jats:sup>\n                    sharing 88.9% average nucleotide identity (ANI) and 38.3% digital DNA\u2013DNA hybridization (dDDH) values with\n                    <jats:italic>R. aureus<\/jats:italic>\n                    , and 81.7% ANI and 25.1% dDDH with\n                    <jats:italic>R. vestalii<\/jats:italic>\n                    , confirming its status as a novel species. Phylogenetic analysis revealed that the \u03b2-galactosidases from these\n                    <jats:italic>Rhodoglobus<\/jats:italic>\n                    strains form a well-supported clade with high sequence similarity. Based on these data, a novel species is proposed:\n                    <jats:italic>Rhodoglobus galactosidasius<\/jats:italic>\n                    sp. nov. (type strain CPHL_1\n                    <jats:sup>T<\/jats:sup>\n                    =ITEM 19986\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 59592\n                    <jats:sup>T<\/jats:sup>\n                    ). Genomic analyses also highlighted its biosynthetic potential for specialized metabolites, consistent with the recognized metabolic diversity of the genus.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007263","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:16:30Z","timestamp":1789661790000},"source":"Crossref","is-referenced-by-count":1,"title":["Rhodoglobus galactosidasius sp. nov., a psychrophilic Actinomycetota producing a cold-active \u03b2-galactosidase isolated from Cape Hallett, Antarctica"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1914-726X","authenticated-orcid":false,"given":"Andrea","family":"Cattaneo","sequence":"first","affiliation":[{"name":"Ca\u2019 Foscari University of Venice, Department of Environmental Sciences, Informatics and Statistics (DAIS), via Torino 155, 30172 Mestre (VE), Italy"},{"name":"National Research Council of Italy (CNR), Institute of Biomolecular Chemistry 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Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9740-4105","authenticated-orcid":false,"given":"Marianna","family":"Pannico","sequence":"additional","affiliation":[{"name":"National Research Council of Italy (CNR), Institute of Polymers, Composites and Biomaterials (IPCB), via Campi Flegrei 34, Pozzuoli, 80078 Napoli (NA), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9590-3816","authenticated-orcid":false,"given":"Barbara","family":"Nicolaus","sequence":"additional","affiliation":[{"name":"National Research Council of Italy (CNR), Institute of Biomolecular Chemistry (ICB), via Campi Flegrei 34, Pozzuoli, 80078 Napoli (NA), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7485-1815","authenticated-orcid":false,"given":"Paola","family":"Di Donato","sequence":"additional","affiliation":[{"name":"Parthenope University of Naples, Department of Science and Technology (DiST), Centro Direzionale - Isola C4, 80143 Napoli (NA), Italy"},{"name":"National Research Council of Italy (CNR), Institute of Biomolecular Chemistry (ICB), via Campi Flegrei 34, Pozzuoli, 80078 Napoli (NA), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6465-4765","authenticated-orcid":false,"given":"Annarita","family":"Poli","sequence":"additional","affiliation":[{"name":"National Research Council of Italy (CNR), Institute of Biomolecular Chemistry (ICB), via Campi Flegrei 34, Pozzuoli, 80078 Napoli (NA), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3606-5140","authenticated-orcid":false,"given":"Ilaria","family":"Finore","sequence":"additional","affiliation":[{"name":"National Research Council of Italy (CNR), Institute of Biomolecular Chemistry (ICB), via Campi Flegrei 34, Pozzuoli, 80078 Napoli (NA), 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