{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:56:06Z","timestamp":1786636566461,"version":"build-2736575974"},"reference-count":54,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["031L0285B"],"award-info":[{"award-number":["031L0285B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]},{"name":"German Technical and Scientific Association","award":["W 201823"],"award-info":[{"award-number":["W 201823"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,13]]},"abstract":"<jats:p>\n                    Four strains of coliform bacteria were isolated from drinking water (strains A2-C1_F and A2-C2_NF) and gastropod samples (strains W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    and S04-F03). 16S rRNA gene sequence analysis showed the highest similarities to members of the genus\n                    <jats:italic>Buttiauxella<\/jats:italic>\n                    (&gt;98%), indicating that all strains are members of this genus. Phylogenomic analyses revealed that strains W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    , S04-F03 and A2-C1_F can be assigned to a putative new\n                    <jats:italic>Buttiauxella<\/jats:italic>\n                    species, while strain A2-C2_NF is a member of the species\n                    <jats:italic>Buttiauxella ferragutiae<\/jats:italic>\n                    . The strains W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    , S04-F03 and A2-C1_F show fluorescence in the Colilert system, indicating unusual\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -\n                    <jats:sc>d<\/jats:sc>\n                    -glucuronidase activity, typically considered indicative for\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (and thus faecal contamination) in water quality analysis. The draft genome of strain W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    has a size of 4.79 Mb and a G+C content of 50.0 mol%. With average nucleotide identity and digital DNA\u2013DNA hybridization values of 96.2 and 68.8%, respectively, strain W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    was most closely related to\n                    <jats:italic>Buttiauxella massiliensis<\/jats:italic>\n                    DSM 110695\n                    <jats:sup>T<\/jats:sup>\n                    . Additional extensive physiological, phenotypic and chemotaxonomic characterization of the strains and all reference strains confirmed the proposal of a novel species. Therefore, the strains W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    , S04-F03 and A2-C1_F represent a novel species for which the name\n                    <jats:italic>Buttiauxella cochleicola<\/jats:italic>\n                    sp. nov. is proposed. The type strain is W03-F01\n                    <jats:sup>T<\/jats:sup>\n                    (=DSM 113920\n                    <jats:sup>T<\/jats:sup>\n                    =CIP 112567\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007265","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:58:24Z","timestamp":1786633104000},"source":"Crossref","is-referenced-by-count":0,"title":["Buttiauxella cochleicola sp. nov., a novel coliform bacterium with \u03b2-d-glucuronidase activity, isolated from gastropods and drinking water"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2820-0333","authenticated-orcid":false,"given":"Michael","family":"H\u00fcgler","sequence":"first","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6940-0024","authenticated-orcid":false,"given":"Carolin","family":"Leister","sequence":"additional","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Aschmann","sequence":"additional","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"B\u00f6sl","sequence":"additional","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Grabner","sequence":"additional","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Kurcz","sequence":"additional","affiliation":[{"name":"TZW: DVGW-Technologiezentrum Wasser, Karlsruher Str. 84, D-76139 Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0692-9410","authenticated-orcid":false,"given":"Sarah","family":"Kirstein","sequence":"additional","affiliation":[{"name":"Leibniz-Institute DSMZ \u2013 German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstra\u00dfe 7B, D-38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9671-5398","authenticated-orcid":false,"given":"Jacqueline","family":"Wolf","sequence":"additional","affiliation":[{"name":"Leibniz-Institute DSMZ \u2013 German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstra\u00dfe 7B, D-38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1105-6363","authenticated-orcid":false,"given":"Richard L.","family":"Hahnke","sequence":"additional","affiliation":[{"name":"Leibniz-Institute DSMZ \u2013 German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstra\u00dfe 7B, D-38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2833-8911","authenticated-orcid":false,"given":"Vera","family":"Thiel","sequence":"additional","affiliation":[{"name":"Leibniz-Institute DSMZ \u2013 German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstra\u00dfe 7B, D-38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","article-title":"Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method; ISO, 9308-2","year":"2012"},{"key":"R2","article-title":"Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora; ISO, 9308-1","year":"2014"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01060-14"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-015-0168-2"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00064-22"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2023.120494"},{"key":"R7","volume-title":"Review of Coliforms as Microbial Indicators of Drinking Water Quality: Recommendations to Change the Use of Coliforms as Microbial Indicators of Drinking Water Quality","author":"Stevens","year":"2003"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.147539"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00471-22"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1002\/9781118960608"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/S0721-9571(81)80016-6"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-1-50"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006334"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-021-02714-3"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-018-1548-5"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.01.025"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3867-3870.2002"},{"key":"R18","first-page":"115","volume-title":"Nucleic Acid Techniques in Bacterial Systematics","author":"Lane","year":"1991"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"R21","volume-title":"Phylogenetic Trees Made Easy.: A How-to Manual, 2nd ed","author":"Hall","year":"2005"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1038\/srep08365"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1105"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1003"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz943"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1080\/10635150802429642"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000760"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac504"},{"key":"R40","article-title":"Identification of bacteria by gas chromatography of cellular fatty acids","author":"Sasser","year":"1990","journal-title":"MIDI Technical Note"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004631"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.7.1467-1470.1989"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1016\/s0163-7827(96)00011-2"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"R45","volume-title":"Methods for General and Molecular Microbiology, 3rd edition","author":"Tindall","year":"2007"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001485"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002516"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R49","first-page":"152","article-title":"Taxonomic parameters revisited: tarnished taxonomic parameters revisited: tarnished gold standards","volume":"2006","author":"Stackebrandt","year":"2006","journal-title":"Microbiol Today"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.09.008"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80028-8"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00715-18"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040023"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv150"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007265?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:58:26Z","timestamp":1786633106000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.007265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":54,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,13]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.007265","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]}}}