{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:29:41Z","timestamp":1787052581766,"version":"build-2736575974"},"reference-count":75,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:p>\n                    A novel halophilic actinobacterium, designated H202\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from a Saharan soil in the Ahb\u00e8s region (B\u00e9ni-Isguen, Gharda\u00efa province, South Algeria), and its taxonomic position was determined by a genome-based polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the strain H202\n                    <jats:sup>T<\/jats:sup>\n                    belonged to the genus\n                    <jats:italic>Actinopolyspora<\/jats:italic>\n                    and showed the highest sequence similarity to\n                    <jats:italic>Actinopolyspora mzabensis<\/jats:italic>\n                    DSM 45460\n                    <jats:sup>T<\/jats:sup>\n                    (99.46%). Comparative genomic analysis based on digital DNA\u2013DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) showed values below the recommended species cut-off thresholds of 70% (dDDH) and 95\u201396% (ANI and AAI), suggesting that the strain H202\n                    <jats:sup>T<\/jats:sup>\n                    represents a novel species. This strain exhibits the typical characteristics of the genus\n                    <jats:italic>Actinopolyspora<\/jats:italic>\n                    . The cell-wall hydrolysates contained\n                    <jats:italic>meso<\/jats:italic>\n                    -diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylcholine was identified as the diagnostic phospholipid, and the predominant menaquinones were MK-9(H\n                    <jats:sub>4<\/jats:sub>\n                    ) and MK-10(H\n                    <jats:sub>4<\/jats:sub>\n                    ). The major fatty acids (\u226510%) were\n                    <jats:italic>anteiso<\/jats:italic>\n                    -C\n                    <jats:sub>17:0<\/jats:sub>\n                    ,\n                    <jats:italic>iso<\/jats:italic>\n                    -C\n                    <jats:sub>15:0<\/jats:sub>\n                    ,\n                    <jats:italic>iso<\/jats:italic>\n                    -C\n                    <jats:sub>16:0<\/jats:sub>\n                    and\n                    <jats:italic>anteiso<\/jats:italic>\n                    -C\n                    <jats:sub>15:0<\/jats:sub>\n                    . The genome size of strain H202\n                    <jats:sup>T<\/jats:sup>\n                    was estimated to be 5.2 Mbp, with a G+C content of 67.5%. Based on these findings, strain H202\n                    <jats:sup>T<\/jats:sup>\n                    is proposed as a representative of a novel species within the genus\n                    <jats:italic>Actinopolyspora<\/jats:italic>\n                    , named\n                    <jats:italic>Actinopolyspora sabaoui<\/jats:italic>\n                    sp. nov. The type strain is H202\n                    <jats:sup>T<\/jats:sup>\n                    (=DSM 46671\n                    <jats:sup>T<\/jats:sup>\n                    =CECT 31287\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007269","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:15:31Z","timestamp":1787051731000},"source":"Crossref","is-referenced-by-count":0,"title":["Actinopolyspora sabaoui sp. nov., a novel halophilic actinobacterium isolated from Gharda\u00efa soil, Algeria"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1197-1107","authenticated-orcid":false,"given":"Rafika","family":"Saker","sequence":"first","affiliation":[{"name":"Laboratoire de Valorisation et Bio-ing\u00e9nierie des Ressources Naturelles (LVBRN), Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Alger 1, Algiers, Algeria"},{"name":"Laboratoire de 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