{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:34:14Z","timestamp":1787762054680,"version":"build-2784847793"},"reference-count":34,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"Prevention and Control of Emerging and Major Infectious Diseases-National Science and Technology Major Project","award":["2025ZD01900100"],"award-info":[{"award-number":["2025ZD01900100"]}]},{"DOI":"10.13039\/100017949","name":"Health Commission of Shanxi Province","doi-asserted-by":"crossref","award":["Grant No. 2024149"],"award-info":[{"award-number":["Grant No. 2024149"]}],"id":[{"id":"10.13039\/100017949","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Young Top-notch Talent Project in the Medical and Health Field of the Sanjin Talents Program","award":["Grant No. SJYC2024469"],"award-info":[{"award-number":["Grant No. SJYC2024469"]}]},{"name":"Youth Fund for Enhancing Capability of Infectious Disease Surveillance and Prevention","award":["Grant No. 102393240020020000003"],"award-info":[{"award-number":["Grant No. 102393240020020000003"]}]},{"name":"Shanxi Provincial Basic Research Program Youth Project","award":["Grant No. 202503021212162"],"award-info":[{"award-number":["Grant No. 202503021212162"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:p>\n                    Four novel bacterial strains representing two novel species were isolated from\n                    <jats:italic>in situ<\/jats:italic>\n                    coal samples. Comparative analysis of 16S rRNA gene sequences showed that strains W1153\n                    <jats:sup>T<\/jats:sup>\n                    and W1478 share the highest sequence similarity with\n                    <jats:italic>Leucobacter tardus<\/jats:italic>\n                    K 70\/01\n                    <jats:sup>T<\/jats:sup>\n                    (98.3%), whereas strains Z1108\n                    <jats:sup>T<\/jats:sup>\n                    and W1038 share the highest sequence similarity with\n                    <jats:italic>L. tardus<\/jats:italic>\n                    K 70\/01\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic>Leucobacter exalbidus<\/jats:italic>\n                    K-540B\n                    <jats:sup>T<\/jats:sup>\n                    (98.0%). Phylogenetic analysis based on 16S rRNA gene sequences and a core-genome phylogeny constructed from 665 core genes demonstrated that the four strains form two distinct clades, clearly separated from other species within the genus\n                    <jats:italic>Leucobacter<\/jats:italic>\n                    . Whole-genome sequencing revealed genome sizes ranging from 2.6 to 2.8 Mb, with G+C contents of 62.5\u201363.5 mol%. The average nucleotide identity and digital DNA\u2013DNA hybridization values between the four strains and all recognized species within the genus\n                    <jats:italic>Leucobacter<\/jats:italic>\n                    ranged from 71.1 to 75.6% and 19.3 to 25.6%, respectively. The major cellular fatty acids of the four strains were anteiso-C\n                    <jats:sub>15 : 0<\/jats:sub>\n                    , anteiso-C\n                    <jats:sub>17 : 0<\/jats:sub>\n                    and iso-C\n                    <jats:sub>16 : 0<\/jats:sub>\n                    . The respiratory quinone types were MK-11 for strain W1153\n                    <jats:sup>T<\/jats:sup>\n                    and MK-9(H8) for strain Z1108\n                    <jats:sup>T<\/jats:sup>\n                    . The main cell-wall sugars were rhamnose and ribose, with 2,4-diaminobutyric acid present as the diagnostic diamino acid. Based on phenotypic characteristics, phylogenetic analyses and chemotaxonomic evidence, strains W1153\n                    <jats:sup>T<\/jats:sup>\n                    and W1478, and Z1108\n                    <jats:sup>T<\/jats:sup>\n                    and W1038, are proposed as representing two novel species within the genus\n                    <jats:italic>Leucobacter<\/jats:italic>\n                    , named\n                    <jats:italic>Leucobacter shiyiweii<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Leucobacter fengyongliangi<\/jats:italic>\n                    sp. nov., respectively. The type strains are W1153\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.5845\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 59606\n                    <jats:sup>T<\/jats:sup>\n                    ) and Z1108\n                    <jats:sup>T<\/jats:sup>\n                    (=GDMCC 1.5846\n                    <jats:sup>T<\/jats:sup>\n                    =KCTC 59607\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007271","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:15:52Z","timestamp":1787760952000},"source":"Crossref","is-referenced-by-count":0,"title":["Leucobacter shiyiweii sp. nov. and Leucobacter fengyongliangi sp. nov., isolated from coal samples in Shanxi Province of China"],"prefix":"10.1099","volume":"76","author":[{"given":"Junyao","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory 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