{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:11:42Z","timestamp":1788973902528,"version":"build-2803163510"},"reference-count":35,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:p>\n                    A novel nitrogen-fixing rhizobial strain, designated Z1P35\u1d40, was isolated from root nodules of\n                    <jats:italic>Pisum sativum<\/jats:italic>\n                    grown in the Za\u00ebr region of Morocco. Phylogenetic analysis of the 16S rRNA gene placed strain Z1P35\u1d40 within the genus\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    , showing 100% sequence identity with several undescribed genospecies of the\n                    <jats:italic>Rhizobium leguminosarum<\/jats:italic>\n                    species complex (Rlc). Strain Z1P35\u1d40 exhibited low average nucleotide identity (ANI) and digital DNA\u2013DNA hybridization (dDDH) values with all described\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    species, but high ANI and dDDH values (97.62 and 78.8%, respectively) with\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    sp. SRDI565, representing genospecies M (GsM) of the Rlc, suggesting that Z1P35\u1d40 represents a novel species corresponding to GsM within this complex. FastANI screening against all\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    genomes available in GenBank revealed that Z1P35\u1d40 shares ANI values above the bacterial species delimitation threshold with 17 unclassified strains, which, together with Z1P35\u1d40 and\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    sp. SRDI565 (GsM), form a distinct lineage within the Rlc. These 17 strains originate from root nodules of diverse legume hosts and are distributed across the Mediterranean region and Australia, including representatives of the symbiovars\n                    <jats:italic>viciae<\/jats:italic>\n                    and\n                    <jats:italic>trifolii<\/jats:italic>\n                    . Phylogenomic analysis further confirms the clustering of Z1P35\u1d40 with\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    sp. SRDI565 (GsM) and several undescribed\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    strains, forming a unique taxonomic unit clearly distinct from other members of the Rlc. Strain Z1P35\u1d40 has a genome of 7.6 Mb with a G+C content of 61 mol% and carries numerous genes associated with chemotaxis, nodulation, nitrogen fixation, phosphate solubilization, iron acquisition and abiotic stress tolerance. Differentiation of Z1P35\u1d40 from described\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    species was further supported by phenotypic and chemotaxonomic analyses. Based on these results, we conclude that Z1P35\n                    <jats:sup>T<\/jats:sup>\n                    belongs to a novel species, corresponding to genospecies M within the Rlc, for which we propose the name\n                    <jats:italic>Rhizobium zaerense<\/jats:italic>\n                    sp. nov. The type strain is Z1P35\u1d40 (DSM 120601\u1d40=CCMM B1365\u1d40).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007277","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:17:32Z","timestamp":1788970652000},"source":"Crossref","is-referenced-by-count":0,"title":["Rhizobium zaerense sp. nov., a novel member of the Rhizobium leguminosarum species complex with a broad geographic distribution and multiple legume hosts"],"prefix":"10.1099","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7003-9734","authenticated-orcid":false,"given":"Mustapha","family":"Missbah El Idrissi","sequence":"first","affiliation":[{"name":"Equipe de Microbiologie et Biologie Mol\u00e9culaire, Centre de Biotechnologies V\u00e9g\u00e9tales et Microbiennes, Biodiversit\u00e9 et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira El","family":"Arraf","sequence":"additional","affiliation":[{"name":"Equipe de Microbiologie et Biologie Mol\u00e9culaire, Centre de Biotechnologies V\u00e9g\u00e9tales et Microbiennes, Biodiversit\u00e9 et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanane","family":"Lamin","sequence":"additional","affiliation":[{"name":"Equipe de Microbiologie et Biologie Mol\u00e9culaire, Centre de Biotechnologies V\u00e9g\u00e9tales et Microbiennes, Biodiversit\u00e9 et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said","family":"Lazar","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences et Techniques de Mohammedia, Universit\u00e9 Hassan II de Casablanca, Mohammedia, 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