{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:03:23Z","timestamp":1789491803386,"version":"build-2803163510"},"reference-count":45,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"the Research on the Pathogenome of Wild Animals","award":["32105"],"award-info":[{"award-number":["32105"]}]},{"name":"the Prevention and Control of Emerging and Major Infectious Diseases-National Science and Technology Major Project","award":["2025ZD01900100; 2025ZD01900110"],"award-info":[{"award-number":["2025ZD01900100; 2025ZD01900110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:p>\n                    Four Gram-stain-positive, aerobic, non-motile and rod-shaped bacterial strains, designated A82\n                    <jats:sup>T<\/jats:sup>\n                    , A588, A196\n                    <jats:sup>T<\/jats:sup>\n                    and A204, were isolated from soil samples collected from\n                    <jats:italic>Marmota sibirica<\/jats:italic>\n                    burrows in Hulun Buir, Nei Mongol Autonomous Region, China. All four strains grew at 4\u201330\u2009\u00b0C, pH 6.0\u201310.0 and 0\u20136.5% (wt\/v) NaCl, and they were positive for oxidase and catalase activities. Phylogenetic analysis of 16S rRNA gene sequences indicated that the four strains belonged to two species within the genus\n                    <jats:italic>Microbacterium<\/jats:italic>\n                    . Strains A82\n                    <jats:sup>T<\/jats:sup>\n                    and A588 exhibited the highest sequence similarity to\n                    <jats:italic>Microbacterium profundi<\/jats:italic>\n                    Shh49\n                    <jats:sup>T<\/jats:sup>\n                    (98.83%) and\n                    <jats:italic>Microbacterium murale<\/jats:italic>\n                    1\u2009Gi-001\n                    <jats:sup>T<\/jats:sup>\n                    (98.67%). Strains A196\n                    <jats:sup>T<\/jats:sup>\n                    and A204 were most closely related to\n                    <jats:italic>Microbacterium aerolatum<\/jats:italic>\n                    NBRC 103071\n                    <jats:sup>T<\/jats:sup>\n                    (99.10%) and\n                    <jats:italic>Microbacterium shaanxiense<\/jats:italic>\n                    CCNWSP60\n                    <jats:sup>T<\/jats:sup>\n                    (98.63%). Comparing the four isolates with their reference strains showed that average nucleotide identity and digital DNA\u2013DNA hybridization values were below the accepted species-delineation benchmarks of 95\u201396\u2009and 70%, respectively. For the type strains A82\n                    <jats:sup>T<\/jats:sup>\n                    and A196\n                    <jats:sup>T<\/jats:sup>\n                    , the DNA G+C content was 64.7 and 65.4\u2009mol%, respectively. Chemotaxonomic analysis revealed that both type strains possessed MK-13 as the sole respiratory quinone. Additionally, diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids were the major polar lipids. Furthermore,\n                    <jats:italic>anteiso<\/jats:italic>\n                    -C\n                    <jats:sub>15\u200a:\u200a0<\/jats:sub>\n                    was identified as the major fatty acid, and the diamino acid in the cell-wall peptidoglycan contained alanine, glutamic acid, glycine, aspartic acid and ornithine. Therefore, strains A82\n                    <jats:sup>T<\/jats:sup>\n                    \/A588 and A196\n                    <jats:sup>T<\/jats:sup>\n                    \/A204 represent two novel species within the genus\n                    <jats:italic>Microbacterium<\/jats:italic>\n                    .\n                    <jats:italic>Microbacterium wangyouchuni<\/jats:italic>\n                    sp. nov. (A82\n                    <jats:sup>T<\/jats:sup>\n                    \/A588, type strain A82\n                    <jats:sup>T<\/jats:sup>\n                    =GDMCC 1.5865\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 38342\n                    <jats:sup>T<\/jats:sup>\n                    ) and\n                    <jats:italic>Microbacterium zouquanmingi<\/jats:italic>\n                    sp. nov. (A196\n                    <jats:sup>T<\/jats:sup>\n                    \/A204, type strain A196\n                    <jats:sup>T<\/jats:sup>\n                    =GDMCC 1.6024\n                    <jats:sup>T<\/jats:sup>\n                    =JCM 38357\n                    <jats:sup>T<\/jats:sup>\n                    ) are proposed.\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.007283","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:16:57Z","timestamp":1789489017000},"source":"Crossref","is-referenced-by-count":0,"title":["Microbacterium wangyouchuni sp. nov. and Microbacterium zouquanmingi sp. nov., isolated from soil in Mongolian marmot (Marmota sibirica) burrows"],"prefix":"10.1099","volume":"76","author":[{"given":"Hongyu","family":"Yang","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and 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